Authors :
Sanaz Aliakbarzadeh; Hossein Ali Khonakdar; Majid Abdouss
Volume/Issue :
Volume 8 - 2023, Issue 12 - December
Google Scholar :
http://tinyurl.com/yc446fr9
Scribd :
http://tinyurl.com/d27vrv88
DOI :
https://doi.org/10.5281/zenodo.10431950
Abstract :
Porous hydrogels can serve as excellent
scaffolds for cartilage tissue regeneration to bridge the
defect gap because if the cartilage is damaged, it has a
limited ability to self-regenerate. Tissue-engineered
scaffolds are often an ideal medium for repairing,
replacing, or regrowing lost or damaged tissue. These
scaffolds act as extracellular matrix (ECM) in nature
and are required to promote cell development and
differentiation. The hydrogel approach is a quick and
effective way to create scaffolds from biocompatible
polymers among scaffold fabrication techniques. In this
research, via synthesis of Gelatin Methacrylate (GelMA)
and Poly(2-Ethyl-2-Oxazoline) (PEtOx) along with
loaded Kartogenin drug (KGN), a porous hydrogel with
an advanced structure was created to regenerate the
extracellular matrix of cartilage. Evaluations were made
on morphology, swelling ratio, degree of hydrophilicity,
weight loss percentage and degradation, elastic modulus,
blood compatibility, drug release, flow cytometry and
MTT. The data revealed that the created hydrogel was
quite porous, with a porosity of 92.2% and linked pores.
With a weight loss of approximately 43% within 14 days,
it was found that the produced hydrogel is
biodegradable at the same time as cell proliferation.
After the stress/strain test, we found that the elastic
modulus of the GelMA/PEtOx hydrogel with the highest
percentage composition and maintaining the structure
and morphology is close to the elasticity modulus of the
cartilage and also the prepared hydrogel showed a good
blood compatibility and sustainable drug release.
According to flow cytometry and MTT tests, up to 75%
of cells can live in this hydrogel and create cell adhesion.
Keywords :
Cartilage, biocompatible hydrogel, Gelatin Methacrylate, Poly(2-Ethyl-2-Oxazoline), Kartogenin.
Porous hydrogels can serve as excellent
scaffolds for cartilage tissue regeneration to bridge the
defect gap because if the cartilage is damaged, it has a
limited ability to self-regenerate. Tissue-engineered
scaffolds are often an ideal medium for repairing,
replacing, or regrowing lost or damaged tissue. These
scaffolds act as extracellular matrix (ECM) in nature
and are required to promote cell development and
differentiation. The hydrogel approach is a quick and
effective way to create scaffolds from biocompatible
polymers among scaffold fabrication techniques. In this
research, via synthesis of Gelatin Methacrylate (GelMA)
and Poly(2-Ethyl-2-Oxazoline) (PEtOx) along with
loaded Kartogenin drug (KGN), a porous hydrogel with
an advanced structure was created to regenerate the
extracellular matrix of cartilage. Evaluations were made
on morphology, swelling ratio, degree of hydrophilicity,
weight loss percentage and degradation, elastic modulus,
blood compatibility, drug release, flow cytometry and
MTT. The data revealed that the created hydrogel was
quite porous, with a porosity of 92.2% and linked pores.
With a weight loss of approximately 43% within 14 days,
it was found that the produced hydrogel is
biodegradable at the same time as cell proliferation.
After the stress/strain test, we found that the elastic
modulus of the GelMA/PEtOx hydrogel with the highest
percentage composition and maintaining the structure
and morphology is close to the elasticity modulus of the
cartilage and also the prepared hydrogel showed a good
blood compatibility and sustainable drug release.
According to flow cytometry and MTT tests, up to 75%
of cells can live in this hydrogel and create cell adhesion.
Keywords :
Cartilage, biocompatible hydrogel, Gelatin Methacrylate, Poly(2-Ethyl-2-Oxazoline), Kartogenin.
