Authors : Dr. Madhavi Jagat; Dr. Pradipkumar Suryawanshi; Dr. Sasmita Tripathy; Dr. Vibha Pali; Dr. Saket Sharma; Dr. Swaranjali Jangde; Dr. Sunita Jain; Dr. Vinit Singh jagat

Volume/Issue : Volume 11 - 2026, Issue 8 - August

Google Scholar : https://tinyurl.com/455eevtd

Scribd : https://tinyurl.com/5waxt7mf

DOI : https://doi.org/10.38124/ijisrt/26aug353

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Background: Oral hygiene is a critical component of general health. Ayurvedic tradition has long advocated the use of medicinal twigs (Dantadhavana) for oral care. Madhuca longifolia (Mahua), classified as the best among Madhura rasa dravyas by Acharya Sushruta, possesses documented antiseptic, antibacterial, and spasmolytic properties.  Objectives: To compare the effectiveness of Madhuca twig with conventional toothbrushing in maintaining oral hygiene, measured by the Oral Hygiene Index (OHI), and to characterise the phytochemical profile of Madhuca twig extract via HPLC-DAD analysis.  Methods: A prospective, non-randomised clinical study was conducted at Shri Khudadaad Dunga ji Government Ayurved College and Hospital, Raipur, enrolling 60 participants (aged 18–50 years) in two groups of 30. Group B used Madhuca twig twice daily; Group A used conventional toothbrush and toothpaste twice daily for 60 days. OHI scores (Debris Index + Calculus Index) were recorded at baseline, Day 15, 30, 45, and 60. HPLC-DAD phytochemical analysis of dried Madhuca twig powder was performed by UltraChrom Innovatives Pvt. Ltd. (ISO 17025 accredited), Wardha.

Keywords : Madhuca Longifolia, Mahua Datun, Dantadhavana, Oral Hygiene Index, HPLC-DAD, Ayurveda, Conventional Brushing, Phytochemical Analysis.

References :

1. Charaka Samhita, Sutra Sthana 30/26. Vidhyotani Tika Hindi commentary, Kashinath Pandey, Goraknath Chaturvedi. Chaukhambha Sanskrit Bhavan, Reprint 2014.
2. Charaka Samhita, Sutra Sthana 5/71–72. Vidhyotani Tika Hindi commentary, Kashinath Pandey, Goraknath Chaturvedi. Chaukhambha Sanskrit Bhavan, Reprint 2014.
3. Sushruta Samhita, Chikitsa Sthana 24. Ayurved Tatva Sandipika by Dr. Ambikadatta Shastri. Chaukhambha Orientalia.
4. Sharma PC, Yelne MB, Dennis TJ. Medicinal Plants Used in Ayurveda, Volume 4. Page 386.
5. World Health Organization. WHO Fact Sheet: Oral Health. Available at: https://www.who.int/health-topics/oral-health
6. Greene JC, Vermillion JR. The Simplified Oral Hygiene Index. J Am Dent Assoc. 1964;68:7–13.
7. UltraChrom Innovatives Pvt. Ltd. HPLC-DAD Interpretation Report: Madhuca longifolia Twig Extract. Wardha, Maharashtra. May 2026. (ISO/IEC 17025:2017 Accredited, Ref No. NA)
8. Arora M. Comparative Study of Khadira Kastha Churna and Madhuka Kastha Churna for Dantadhavana Upkrama in Maintenance of Oral Hygiene. Pune, Maharashtra, 2019.