The present study carried out to determine the comparative study of antibacterial activity of epidermal secreations of two fishes channa punctatus and Hetetropneustes fossilis, against bacterial strains. i.e Escherchia coli, Klebsiella pneumonia, Staphylococcus aereus, Proteus vulgaris. In Channa punctatus higest Zone of inhibition occurs with Staphylococcus aureus (30mm) and minimum ZOI occurs with Klebsiella pneumoniae (19mm) . In heteropneustes fossilis the higest Zone of inhibition occurs Escherchia coli (30mm)., minimum ZOI with Klebsiella pneumonia (20mm) . The antibacterial activity of epidermal mucus secretion was compared between two fishes channa punctatus and Hetetropneustes fossilis .

Keywords : Antibacterial, fish mucus, Channa punctatus, Hateropneustes fossilis Epidermal mucus Pathogen, Zone of inhibition.