Authors : Mary Lawrence Samidapogu

Volume/Issue : Volume 10 - 2025, Issue 6 - June

Google Scholar : https://tinyurl.com/yfhjddsa

DOI : https://doi.org/10.38124/ijisrt/25jun393

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Chemical profiling serves as a key technique in analytical chemistry, focusing on the identification, quantification, and characterization of chemical components in complex mixtures. Mass spectrometry (MS), renowned for its outstanding sensitivity, specificity, and capability to analyze compounds with a diverse range of molecular weights and polarities, has emerged as one of the most effective instruments for chemical profiling. This extensive study examines the basic principles of mass spectrometry, its combination with chromatographic techniques, and the processes involved in sample preparation, ionization, and detection. Different ionization methods such as Electrospray Ionization (ESI), Matrix-Assisted Laser Desorption Ionization (MALDI), and Atmospheric Pressure Chemical Ionization (APCI) are examined, along with mass analyzers like Quadrupole, Time-of-Flight (TOF), Orbitrap, and Ion Trap analyzers. The research also explores advanced configurations of mass spectrometry, including tandem mass spectrometry (MS/MS) and high-resolution mass spectrometry (HRMS), which have greatly improved both the qualitative and quantitative capabilities of the technique. By facilitating accurate molecular identification and fingerprinting of unknown substances, MS aids in essential decision-making processes within both research and regulatory contexts. However, despite its benefits, mass spectrometry encounters challenges like matrix effects, significant operational expenses, and the requirement for skilled personnel. This comprehensive study aims to explore the principles, methodologies, and applications of chemical profiling using mass spectrometry. It presents an in- depth analysis of instrumentation, ionization strategies, separation techniques, and real-world applications, while also addressing limitations and emerging solutions. By consolidating the current state of knowledge and forecasting future trends, this work highlights the central role of MS in shaping the future of chemical and biochemical analysis.

Keywords : Chemical Profiling, Mass-to-Charge Ratio (m/z), Chromatographic Coupling, High-Resolution Mass Spectrometry (HRMS), Tandem Mass Spectrometry (MS/MS), Proteomics and Metabolomics.

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