Typhoid fever leads to a major consequence, the intestinal perforation, which occurs to the very infected patients. The intestinal perforation remains one of the most complication of this disease in developing countries. To share responsibility with the surgeon who declares this perforation and repairs the damage, this study is performed, not only to isolate the Salmonella enterica subspecie, enterica serovar Typhi strains, but also to detect their DNA fragment in peritoneal fluid, collected from peritoneal cavity of the surgically operated patients. To attain this goal, the peritoneal fluid collected from some hospital of the City of Kinshasa, the Democratic Republic of the Congo (R.D.C.)has been carried to the National Institute for Biomedical Research (INRB)in Kinshasaand at Kenya Medical Research Institute (KEMRI) in Nairobi, respectively, to perform bacterial culture for the Salmonella Typhi isolation and to detect its DNA. Of 28 patients who have been operated with ileal perforation, associated to typhoid fever, 71.4% were male with a median age of 20.5 years. This prospective study, performed by culture of peritoneal fluid in MacConkey agar and by polymerase chain reaction in the same specimen did not, neither isolate the Salmonella Typhi nor detect its DNA.

Keywords : Intestinal perforation, peritoneal fluid, Isolation, Salmonella Typhi, INRB, Kinshasa, D.R. C, Detection, DNA, KEMRI.