Authors :
Dr. G. Abirami; Dr. T. Vetrichelvan; M. Raman; S. Suvetha
Volume/Issue :
Volume 8 - 2023, Issue 3 - March
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://bit.ly/3M6FjkP
DOI :
https://doi.org/10.5281/zenodo.7789308
Abstract :
Three simple, accurate, novel, safe and
precise methods were developed by the selective UV
method for the estimation of water-soluble drug
Prucalopride Succinate in pharmaceutical Dosage Form.
Estimation was achieved by Shimadzu -1700 Double
Beam UV Visible Spectrophotometer with a pair of
10mm matched quartz cells and ELICO SL -210 Double
Beam UV Spectrophotometer with a pair of 10mm
matched quartz cells. By using UV Spectrophotometer,
standard solution was absorbed. From the UV spectra
obtained, Prucalopride Succinate showed maximum
absorbance at 226nm. By using Visible
Spectrophotometer, standard solution was absorbed
with 2% Ferric chloride and MBTH reagent. From the
Visible spectrum obtained, 483nm was selected as
maximum absorbance for the analysis of Prucalopride
succinate.The method was validated for specificity,
linearity, precision, accuracy, robustness. The linearity
of the proposed methods of UV Spectrophotometer, first
order derivative spectrophotometer,Visible
Spectrophotometer was at the range of 3-18µg/ml, 10-
60µg/ml, 100µg/mlof Prucalopride succinate with a
correlation coefficient of 0.9999. The precision (relative
standard deviation – RSD) of six samples was 0.5066%.
The accuracy (recovery) was 100.07 % and 100.19
%.and 99.93%.
Keywords :
UV Spectrophotometer, Visible Spectrophotometer, Prucalopride succinate, RSD and Validation
Three simple, accurate, novel, safe and
precise methods were developed by the selective UV
method for the estimation of water-soluble drug
Prucalopride Succinate in pharmaceutical Dosage Form.
Estimation was achieved by Shimadzu -1700 Double
Beam UV Visible Spectrophotometer with a pair of
10mm matched quartz cells and ELICO SL -210 Double
Beam UV Spectrophotometer with a pair of 10mm
matched quartz cells. By using UV Spectrophotometer,
standard solution was absorbed. From the UV spectra
obtained, Prucalopride Succinate showed maximum
absorbance at 226nm. By using Visible
Spectrophotometer, standard solution was absorbed
with 2% Ferric chloride and MBTH reagent. From the
Visible spectrum obtained, 483nm was selected as
maximum absorbance for the analysis of Prucalopride
succinate.The method was validated for specificity,
linearity, precision, accuracy, robustness. The linearity
of the proposed methods of UV Spectrophotometer, first
order derivative spectrophotometer,Visible
Spectrophotometer was at the range of 3-18µg/ml, 10-
60µg/ml, 100µg/mlof Prucalopride succinate with a
correlation coefficient of 0.9999. The precision (relative
standard deviation – RSD) of six samples was 0.5066%.
The accuracy (recovery) was 100.07 % and 100.19
%.and 99.93%.
Keywords :
UV Spectrophotometer, Visible Spectrophotometer, Prucalopride succinate, RSD and Validation
