Authors : Ishmeal Kwaku Duah

Volume/Issue : Volume 10 - 2025, Issue 9 - September

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Google Scholar : https://tinyurl.com/2zn44v56

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DOI : https://doi.org/10.38124/ijisrt/25sep1355

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Abstract : These CRISPR-Cas biosensors offer high specificity due to guide RNA programming and exceptional sensitivity often boosted by isothermal amplification, with multiple readout options. This review highlights recent progress in using CRISPR-Cas biosensors to detect vital water pollutants, such as pathogenic microbes, toxins, heavy metals, and organic micropollutants. It examines how these biosensors have transitioned from lab prototypes to field-ready devices, evaluates their effectiveness across various water samples, and discusses the challenges and future opportunities for this innovative technology. The aim is to provide readers with a current overview of the latest advances in CRISPR-Cas biosensor systems, deepen their understanding, and inspire further development of portable CRISPR-Cas technologies.

Keywords : CRISPR-Cas Biosensors, Water Pollutants and Contaminants, Bacteria, Viruses, Heavy Metals, Biotoxins.

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