Bloodstream infection is an infectious disease characterized by the presence of live bacterial or fungal microorganisms in the bloodstream, as evidenced by positive blood cultures. These pathogens have an important role in infection, especially in the intensive care unit (ICU). Although infection is not the most common cause of mortality in ICU care, infection is confirmed to be associated with increased hospitalization and high treatment costs. Objective: This study aims to determine the profile of blood culture, the factors that have a bearing on the results, and the description of antibiotic resistance in patients at Haji Adam Malik General Hospital. Method: This study is a study using secondary data from patients in ICU Haji Adam Malik General Hospital in 2019 - 2020 conducted by blood culture. Results: This study included data from 52 patients who were recorded between 2019 – 2020 and underwent blood culture. There are 50% women and men each with an average age of 54,69 years. Based on the acquisition of a positive blood culture in terms of the day of admission, found 33 people (61.4%) positive patients after passing 48 hours. The highest morphology was Gram negative rods, 52.8% with the most species, Klebsiella pneumoniae ESBL positive, and found Carbapenemase-producing bacteria (5.5%). The most common Gram-positive bacterium is Staphylococcus aureus (15%), and half is suspected MRSA. Conclusion: Most types of bacteria found in ICU patients in Haji Adam Malik General Hospital are Gramnegative rod bacteria, with the time it was found above 48 hours after entering the hospital. The picture of antibiotic resistance is in line with the bacteria found with the property of drug resistance. Preventive measures are needed to prevent patients from the possibility of transmitting these microorganisms

Keywords : Culture, Blood, Microorganisms, Resistance, ICU