Authors :
Dr. Pranab Karmaker, Dr. Abu Hena Mostafa Kamal, Dr. Arup Kumar Saha, Dr. Ariful Haque
Volume/Issue :
Volume 4 - 2019, Issue 12 - December
Google Scholar :
https://goo.gl/DF9R4u
Scribd :
https://bit.ly/2EWYLwg
Abstract :
Background:
β-Lactam antibiotics are commonly used to treat
bacterial infections. The groups of antibiotics in this
catagory include penicillins, cephalosporins,
carbapenems & monobactams. Increased use of
antibiotics, particularly the third generation of
cephalosporins, has been associated with the emergence
of β-Lactamases mediated bacterial resistance, which
subsequently led to the development of ESBL producing
bacteria. ESBL screening as a routine test has not yet
been practiced in Bangladesh. So, main objective is to
determine the extended spectrum beta lactamase
(ESBL) among Escherichia coli, Klebsiella spp. and
other strains and to detect main ESBL and NDM
producing genes by PCR.
Materials/Methods:
Patients with Bacterial infection. UTI reporting,
PUS, RTI and Wound infection in RMCH. These
sample was cultured in a MacConkey agar. Disk
diffusion and Double disk diffusion test was done.
Results:
Among 1423 meropenem resistant isolates, the
breakup was, Klebsiella sp - 381, Proteus sp - 176, Esch.
coli - 189, Pseudomonas sp - 119, Others - 147. In the
present study, test strains showed potentiality against
higher classes of antibiotics such as carbapenem group.
After PCR we found NDM-1 positive isolate.
Conclusions:
The results shows that blaNDM-1 is the most
frequent gene identified in most of the isolates. blaNDM-1
appear to be an emerging cause of carbapenem
resistance in enterobacteriaceae.
Keywords :
Enterobacteriaceae, NDM-1, ESBLs.
Background:
β-Lactam antibiotics are commonly used to treat
bacterial infections. The groups of antibiotics in this
catagory include penicillins, cephalosporins,
carbapenems & monobactams. Increased use of
antibiotics, particularly the third generation of
cephalosporins, has been associated with the emergence
of β-Lactamases mediated bacterial resistance, which
subsequently led to the development of ESBL producing
bacteria. ESBL screening as a routine test has not yet
been practiced in Bangladesh. So, main objective is to
determine the extended spectrum beta lactamase
(ESBL) among Escherichia coli, Klebsiella spp. and
other strains and to detect main ESBL and NDM
producing genes by PCR.
Materials/Methods:
Patients with Bacterial infection. UTI reporting,
PUS, RTI and Wound infection in RMCH. These
sample was cultured in a MacConkey agar. Disk
diffusion and Double disk diffusion test was done.
Results:
Among 1423 meropenem resistant isolates, the
breakup was, Klebsiella sp - 381, Proteus sp - 176, Esch.
coli - 189, Pseudomonas sp - 119, Others - 147. In the
present study, test strains showed potentiality against
higher classes of antibiotics such as carbapenem group.
After PCR we found NDM-1 positive isolate.
Conclusions:
The results shows that blaNDM-1 is the most
frequent gene identified in most of the isolates. blaNDM-1
appear to be an emerging cause of carbapenem
resistance in enterobacteriaceae.
Keywords :
Enterobacteriaceae, NDM-1, ESBLs.
