Analysis of Multidrug Resistant NDM Producing Bacteria in ICU

Authors : Dr. Pranab Karmaker, Dr. Abu Hena Mostafa Kamal, Dr. Arup Kumar Saha, Dr. Ariful Haque

Volume/Issue : Volume 4 - 2019, Issue 12 - December

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 Background: β-Lactam antibiotics are commonly used to treat bacterial infections. The groups of antibiotics in this catagory include penicillins, cephalosporins, carbapenems & monobactams. Increased use of antibiotics, particularly the third generation of cephalosporins, has been associated with the emergence of β-Lactamases mediated bacterial resistance, which subsequently led to the development of ESBL producing bacteria. ESBL screening as a routine test has not yet been practiced in Bangladesh. So, main objective is to determine the extended spectrum beta lactamase (ESBL) among Escherichia coli, Klebsiella spp. and other strains and to detect main ESBL and NDM producing genes by PCR.  Materials/Methods: Patients with Bacterial infection. UTI reporting, PUS, RTI and Wound infection in RMCH. These sample was cultured in a MacConkey agar. Disk diffusion and Double disk diffusion test was done.  Results: Among 1423 meropenem resistant isolates, the breakup was, Klebsiella sp - 381, Proteus sp - 176, Esch. coli - 189, Pseudomonas sp - 119, Others - 147. In the present study, test strains showed potentiality against higher classes of antibiotics such as carbapenem group. After PCR we found NDM-1 positive isolate.  Conclusions: The results shows that blaNDM-1 is the most frequent gene identified in most of the isolates. blaNDM-1 appear to be an emerging cause of carbapenem resistance in enterobacteriaceae.

Keywords : Enterobacteriaceae, NDM-1, ESBLs.


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