Authors :
KrunalPawar; Dr. Siddhivinayak Barve; Dr. Neeta S. Madan
Volume/Issue :
Volume 7 - 2022, Issue 4 - April
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3wu9DfG
DOI :
https://doi.org/10.5281/zenodo.6562573
Abstract :
Even though jatropha holds enormous
potential as a biodiesel feedstock, there are still significant
obstacles that can be overcome before it can be
economically viable in many parts of the world. Jatropha
curcas develops anthracnose due to
Colletotrichumgloeosporioides and it affects each
genotype in a different way. A gene-expression profiling
study was performed with total RNA from two cultivars
of Jatropha curcas that had and did not have a
Colletotrichumgloeosporioides inoculation line in order to
identify potential candidate genes involved in pathogenplant interactions. There were 213 significant differential
gene expressions between the wild type 9-1 and RJ127
cultivar lines inoculated with
Colletotrichumgloeosporioides. A comprehensive analysis
of these genes provided information regarding the
signaling system, hormone biosynthesis and regulation,
transcription regulation, and ubiquitin-mediated
proteolysis. Gene Ontology (GO:0006952) annotations
were found for nine genes associated with defense
response, six orthologous genes were associated with
pathogen-plant interactions, and 27 transcription factors
were associated with fungus responses. The expression
levels of genes varied among cultivars when infected with
Colletotrichumgloeosporioides, according to gene
expression analysis. Transcriptome analyses were used to
diagnose candidate genes for transcription factors and
structural genes that may be involved in plant-pathogen
interactions in Jatropha curcas leaves of the cultivated
genotypes. These analyses provided new insights into the
defense mechanisms controlling infection associated with
cultivar Jatropha curcas 9-1. The structural genes and
regulators identified here can also be used as efficient
genetic markers for the selection of cultivars with
excessive defense mechanisms.
Keywords :
Jatropha; C. gloeosporioides; NGS; RNA-Seq; DGEs; biodiesel; NGS
Even though jatropha holds enormous
potential as a biodiesel feedstock, there are still significant
obstacles that can be overcome before it can be
economically viable in many parts of the world. Jatropha
curcas develops anthracnose due to
Colletotrichumgloeosporioides and it affects each
genotype in a different way. A gene-expression profiling
study was performed with total RNA from two cultivars
of Jatropha curcas that had and did not have a
Colletotrichumgloeosporioides inoculation line in order to
identify potential candidate genes involved in pathogenplant interactions. There were 213 significant differential
gene expressions between the wild type 9-1 and RJ127
cultivar lines inoculated with
Colletotrichumgloeosporioides. A comprehensive analysis
of these genes provided information regarding the
signaling system, hormone biosynthesis and regulation,
transcription regulation, and ubiquitin-mediated
proteolysis. Gene Ontology (GO:0006952) annotations
were found for nine genes associated with defense
response, six orthologous genes were associated with
pathogen-plant interactions, and 27 transcription factors
were associated with fungus responses. The expression
levels of genes varied among cultivars when infected with
Colletotrichumgloeosporioides, according to gene
expression analysis. Transcriptome analyses were used to
diagnose candidate genes for transcription factors and
structural genes that may be involved in plant-pathogen
interactions in Jatropha curcas leaves of the cultivated
genotypes. These analyses provided new insights into the
defense mechanisms controlling infection associated with
cultivar Jatropha curcas 9-1. The structural genes and
regulators identified here can also be used as efficient
genetic markers for the selection of cultivars with
excessive defense mechanisms.
Keywords :
Jatropha; C. gloeosporioides; NGS; RNA-Seq; DGEs; biodiesel; NGS
