A simple, accurate, rapid and precise isocratic reversed phase high-performance liquid chromatographic method has been developed and validated for simultaneous determination of Gabapentin, Hesperidine and Fosphenytoin sodium in tablet. The chromatographic separation was carried out on an waters C18 analytical column (150×4.6 mm; 5 μm) with a mixture of water:acetonitrile pH 7.0 adjusted with ophosphoric acid (80:20, v/v) as mobile phase; at a flow rate of 1 ml/min. UV detection was performed at 214 nm. The retention times were 5.2, 7.9 and 16.9 min. for Gabapentin, Hesperidine and Fosphenytoin sodium, respectively. Calibration plots were linear (r2>0.999) over the concentration range 60-180 μg/ml for all three drugs. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. Validation studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of Gabapentin, Hesperidine and Fosphenytoin sodium in bulk drug and tablet dosage form.
Key words: Gabapentin, Hesperidine and Fosphenytoin