Authors :
Regina Septiana Barung; Boy Rahardjo Sidharta; P. Kianto Atmodjo
Volume/Issue :
Volume 8 - 2023, Issue 12 - December
Google Scholar :
http://tinyurl.com/5n942w6z
Scribd :
http://tinyurl.com/3x6tfp96
DOI :
https://doi.org/10.5281/zenodo.10473992
Abstract :
Ethanolic extract of senggani (Melastoma
malabathricum L.) leaf contains secondary metabolites
such as flavonoids, tannins, steroids, alkaloids, saponins,
and triterpenoids. Flavonoids which are dominant
among other compounds have shown antibacterial
activity, especially in Pseudomonas aeruginosa and
Staphylococcus aureus. The research aims to determine
the inhibition zone and minimum inhibition
concentration (MIC) of the leaf extract against P.
aeruginosa and S. aureus. The leaf extract is done by
maceration utilizing 70 % ethanol (pa) and variation of
concentrations viz. 5, 10, and 15 % were treated to P.
aeruginosa and S. aureus. The positive control is
ampicillin, and the negative control is the solvent
(ethanol 70 %). The results show that the leaf extract at
15% shows the highest inhibition zone against P.
aeruginosa and S. aureus at 0.63±0.18 and 1.48±0.47 cm2
,
respectively. The MIC for P. aeruginosa is 15% and for S.
aureus is 10%.
Keywords :
Melastoma malabathricum, antibacterial activity, MIC, skin infection
Ethanolic extract of senggani (Melastoma
malabathricum L.) leaf contains secondary metabolites
such as flavonoids, tannins, steroids, alkaloids, saponins,
and triterpenoids. Flavonoids which are dominant
among other compounds have shown antibacterial
activity, especially in Pseudomonas aeruginosa and
Staphylococcus aureus. The research aims to determine
the inhibition zone and minimum inhibition
concentration (MIC) of the leaf extract against P.
aeruginosa and S. aureus. The leaf extract is done by
maceration utilizing 70 % ethanol (pa) and variation of
concentrations viz. 5, 10, and 15 % were treated to P.
aeruginosa and S. aureus. The positive control is
ampicillin, and the negative control is the solvent
(ethanol 70 %). The results show that the leaf extract at
15% shows the highest inhibition zone against P.
aeruginosa and S. aureus at 0.63±0.18 and 1.48±0.47 cm2
,
respectively. The MIC for P. aeruginosa is 15% and for S.
aureus is 10%.
Keywords :
Melastoma malabathricum, antibacterial activity, MIC, skin infection
