This study was carried out to isolate and determine the antibiogram of Staphylococcus species from Cow droppings in Ogun state. A total of sixty droppings were collected from a livestock farm in Ogere, Ogun state, and thirty-five Staphylococcus species were isolated from the samples collected. Antibiotic susceptibility testing using the disc diffusion method was done using antibiotics such as Ciprofloxacin, Imipenem, Tazobactam, and Amoxicillin and results were interpreted as described by the Clinical Laboratory Standards Institute (CLSI, 2021). The Isolated Staphylococcus species were 100% sensitive to Imipenem while the isolates were 89% resistant to Amoxicillin, 20% resistant to Tazobactam, and 3% resistant to Ciprofloxacin. Cow droppings can pose a public health risk and cause contamination to the environment and also to man because organisms isolated can become pathogenic organisms and so therefore improved farm hygiene and also the use of effective antibiotics to help reduce mortality associated with infectious diseases caused by pathogenic Staphylococci species frow the cow droppings should be practiced.

Keywords : Cow Droppings, Staphylococcus Species, Antibiogram.