Authors :
Nonye T. Ujam; Princeton Shekinah; Cyril C. Adonu; Amaka H. Okorie; Restus Onwusaba; Chidimma R. Chukunwejim; Emmanuel Ogudu; Chizoba A. Ozochi; Cornelius Ozochikelu; Christian C. Aneke; Luke J. Ugwu
Volume/Issue :
Volume 11 - 2026, Issue 6 - June
Google Scholar :
https://tinyurl.com/z62ccmyh
Scribd :
https://tinyurl.com/2w3vjs99
DOI :
https://doi.org/10.38124/ijisrt/26jun1045
Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.
Abstract :
Diabetic foot infection is a serious complication associated with diabetes. The condition affects the quality of life
in patients of all classes, races, and ages. If left untreated, may lead to gangrene, osteomyelitis, and amputation. This study
was conducted to ascertain the antibiotic susceptibility profiles of bacteria from diabetic foot infections (DFIs) in Enugu.
Demographical and clinical history data were gathered using structured questionnaire. Diabetic wound samples were
collected and analysed using standard microbiological procedures. Isolation and identification of bacterial species were
based on cultural morphology, microscopy, and biochemical tests. Antibiotic susceptibility was assessed on eight antibiotic
agents: amoxycillin/clavulanic acid, nitrofurantoin, cefoxitin, imipenem, gentamicin, ciprofloxacin, colistin sulphate, and
cotrimoxazole following the Kirby-Bauer disc diffusion technique and CLSI guidelines.
Keywords :
Diabetes, Diabetic Foot Infection, Antibiotic Resistance, Foot Ulcer, Polymicrobial.
References :
- Aamer, A., Boughalem, Y., Kamouni, Y.E., Arsalane, L. and Zouhair, S. (2025). Microbiological profile and antibiotic resistance in diabetic foot infections: a retrospective study from Marrakech. GSC Biological and Pharmaceutical Sciences, 33(1), 246–251. https://doi.org/10.30574/gscbps.2025.33.1.0411
- Agbo, I.U., Udeani, T.K. and Ngwu, M.A. (2025). Prevalence of extended spectrum beta-lactamase producing bacteria in patients with wound infections attending tertiary hospitals in Enugu, Nigeria. South Asian Journal of Research in Microbiology, 19(2), 1–13. https://doi.org/10.9734/sajrm/2025/v19i2416
- Atlaw, A., Kebede, H.B., Abdela, A.A. and Woldeamanuel, Y. (2022). Bacterial isolates from diabetic foot ulcers and their antimicrobial resistance profile from selected hospitals in Addis Ababa, Ethiopia. Frontiers in Endocrinology, 13(8), 1–10. https://doi.org/10.3389/fendo.2022.987487
- Chaves, C.R.S., Salamandane, C., Maurício, B.S., Machamba, A.A.L., Salamandane, A. and Brito, L. (2025). Multidrug-resistant staphylococcus aureus in diabetic foot infections (DFI) from Beira, Mozambique: Prevalence and virulence profile. Infection and Drug Resistance, 18(5), 2779–2796. https://doi.org/10.2147/IDR.S521876
- CLSI. (2024). Performance Standards for Antimicrobial Susceptibility Testing. (CLSI, Ed.), CLSI supplement M100 (34th Editi). Wayne, PA: USA: CLSI. Retrieved from http://www.iacld.ir/DL/public/CLSI-2018-M100-S28.pdf
- Coşkun, B., Ayhan, M., Ulusoy, S., & Guner, R. (2024). Bacterial Profile and Antimicrobial Resistance Patterns of Diabetic Foot Infections in a Major Research Hospital of Turkey. Antibiotics, 13(7). https://doi.org/10.3390/antibiotics13070599
- Das, L., Smriti, S., Dash, R.K., Singh, N., Panda, S.S., Mohapatra, I., Moharana, N. and Mulla, B., Das, S. and Pattnaik, D. (2025). Clinico microbiological profile of diabetic foot ulcers: A retrospective study. Cureus, 17(10), e95291. https://doi.org/10.7759/CUREUS.95291
- Du, F., Ma, J., Gong, H., Bista, R., Zha, P., Ren, Y., … Wang, C. (2022). Microbial infection and antibiotic susceptibility of diabetic foot ulcer in China: Literature review. Frontiers in Endocrinology, 13(5), 1–9. https://doi.org/10.3389/fendo.2022.881659
- Genitsaridi, I., Salpea, P., Salim, A., Sajjadi, S. F., Tomic, D., James, S., … Magliano, D. J. (2026). 11th edition of the IDF diabetes atlas: Global, regional, and national diabetes prevalence estimates for 2024 and projections for 2050. The lancet. Diabetes and Endocrinology, 14(2), 149–156. https://doi.org/10.1016/S2213-8587(25)00299-2
- Glen, K. A. and Lamont, I. L. (2021). β-lactam resistance in Pseudomonas aeruginosa: Current status, future prospects. Pathogens, 10(12), 1638. https://doi.org/10.3390/PATHOGENS10121638/S1
- Goh, T. C., Bajuri, M. Y., Nadarajah, S. C., Rashid, A. H. A., Baharuddin, S. and Zamri, K. S. (2020). Clinical and bacteriological profile of diabetic foot infections in a tertiary care. Journal of Foot and Ankle Research, 13(1), 1–8.
- Govindaswamy, S., Subbarayan, E., Jaishwanth, V. M., Kumar, S. S. A., Santhosh, S. and Renji, S. A. (2025). Bacteriological profile and antimicrobial susceptibility patterns in patients with diabetic foot infections in a tertiary care hospital in South India. Journal of Pure and Applied Microbiology, 19(3), 1948–1954. https://doi.org/10.22207/JPAM.19.3.21
- Guo, H., Song, Q., Mei, S., Xue, Z., Li, J. and Ning, T. (2023). Distribution of multidrug-resistant bacterial infections in diabetic foot ulcers and risk factors for drug resistance: A retrospective analysis. PeerJ, 11. https://doi.org/10.7717/peerj.16162
- Ismayıl, A., Colak, B., Yormaz, S., Turk Dagi, H. and Macin, S. (2025). Investigation of causing microorganisms and antimicrobial sensitivity patterns in diabetic foot wounds. Genel Tip Dergisi, 35(2), 276–284. https://doi.org/10.54005/geneltip.1586166
- Karimi, M. A., Binaei, S., Hashemi, S. H., Refahi, P., Olama, E., Olama, E., … Deravi, N. (2024). Marital status and risk of type 2 diabetes among middle-aged and elderly population: a systematic review and meta-analysis. Frontiers in Medicine, 11(1). https://doi.org/10.3389/fmed.2024.1485490
- Li, W., Sadeh, O., Chakraborty, J., Yang, E., Basu, P. and Kumar, P. (2025). Multifaceted antibiotic resistance in diabetic foot infections: A systematic review. Microorganisms, 13(10), 1–19. https://doi.org/10.3390/microorganisms13102311
- Matta-Gutiérrez, G., García-Morales, E., García-álvarez, Y., Álvaro-Afonso, F. J., Molines-Barroso, R. J. and Lázaro-Martínez, J. L. (2021). The influence of multidrug-resistant bacteria on clinical outcomes of diabetic foot ulcers: A systematic review. Journal of Clinical Medicine, 10(9). https://doi.org/10.3390/jcm10091948
- Namatovu, A., Vahwere, B. M., Ntulume, I., Ssebuufu, R., Nalubega, R., Pius, T., … Nasinyama, G. W. (2025). Antimicrobial resistance profiles and detection of mecA, blaCTX-M-1, and blaSHV genes in bacteria among diabetic foot ulcer patients from selected referral hospitals in Uganda. BMC Infectious Diseases, 25(1). https://doi.org/10.1186/s12879-025-11909-z
- O’Neill, J. (2015). Antimicrobials in agriculture and the environment: Reducing unnecessary use and waste. The Review on Antimicrobial Resistance, (12). https://doi.org/Medium
- Ozochi, C. A., Ibangha, I. A. I., Onuora, S. O., Nwankwo, C. E. I., Enebe, M. C., Adukwu, E. C., … Chigor, V. N. (2025). Prevalence and characterization of antibiotic resistance genes of Vibrio species from Asata River, Enugu, Southeast Nigeria. Microbe, 7(4), 100349. https://doi.org/10.1016/j.microb.2025.100349
- Saltoglu, N., Ergonul, O., Tulek, N., Yemisen, M., Kadanali, A., Karagoz, G., … Sargın, F. (2018). Influence of multidrug resistant organisms on the outcome of diabetic foot infection. International Journal of Infectious Diseases, 70, 10–14. https://doi.org/10.1016/j.ijid.2018.02.013
- Tarigan, M. B., Saragih, R. M., Tarigan, K. A. and Ginting, F. (2025). Antimicrobial resistance and empirical antibiotic use in diabetic foot infections: A retrospective study from Indonesia. Narra J, 5(3), e2895. https://doi.org/10.52225/narra.v5i3.2895
- Tola, A., Regassa, L. D. and Ayele, Y. (2021). Prevalence and associated factors of diabetic foot ulcers among type 2 diabetic patients attending chronic follow-up clinics at governmental hospitals of Harari Region, Eastern Ethiopia: A 5-year (2013–2017) retrospective study. SAGE Open Medicine, 9. https://doi.org/10.1177/2050312120987385
- Ugwu, O., Udeani, T. K., Anigbo, C. and Anigbo, C. (2024). Detection of microbial pathogens colonizing foot ulcers of diabetic patients in Enugu, Nigeria. African Journal of Clinical and Experimental Microbiology, 25(2), 169–180.
- Winski, R., Xu, J., Chan, A., Wattengel, B. A., Puckett, A., Huntsman, K., … Mergenhagen, K. A. (2025). Microbiology of diabetic foot infections in U.S. Veterans. Clinical Diabetes, 43(3), 366–370. https://doi.org/10.2337/CD24-0086
- Yan, X., Song, J. fang, Zhang, L. and Li, X. (2022). Analysis of risk factors for multidrug-resistant organisms in diabetic foot infection. BMC Endocrine Disorders, 22(1), 1–7. https://doi.org/10.1186/s12902-022-00957-0
- Yang, S., Hu, L., Zhao, Y., Meng, G., Xu, S. and Han, R. (2024). Prevalence of multidrug-resistant bacterial infections in diabetic foot ulcers: A meta-analysis. International Wound Journal, 21(4), 1–14. https://doi.org/10.1111/iwj.14864
- Zambelli, R., Santos, A. F., Moreira, L. R., Ribeiro, H. M., Simões, R., Magalhães, J. M., … Leopoldino, A. O. (2025). Bacterial profile and antimicrobial resistance in diabetic foot ulcer infections: a 10-year retrospective cohort study. Brazilian Journal of Infectious Diseases, 29(5). https://doi.org/10.1016/j.bjid.2025.104570
Diabetic foot infection is a serious complication associated with diabetes. The condition affects the quality of life
in patients of all classes, races, and ages. If left untreated, may lead to gangrene, osteomyelitis, and amputation. This study
was conducted to ascertain the antibiotic susceptibility profiles of bacteria from diabetic foot infections (DFIs) in Enugu.
Demographical and clinical history data were gathered using structured questionnaire. Diabetic wound samples were
collected and analysed using standard microbiological procedures. Isolation and identification of bacterial species were
based on cultural morphology, microscopy, and biochemical tests. Antibiotic susceptibility was assessed on eight antibiotic
agents: amoxycillin/clavulanic acid, nitrofurantoin, cefoxitin, imipenem, gentamicin, ciprofloxacin, colistin sulphate, and
cotrimoxazole following the Kirby-Bauer disc diffusion technique and CLSI guidelines.
Keywords :
Diabetes, Diabetic Foot Infection, Antibiotic Resistance, Foot Ulcer, Polymicrobial.