This study assessed the antibiotic susceptibility
pattern of bacterial isolates from selected boreholes and
hand-dug wells water in Senior Staff Quarters, Obafemi
Awolowo University, Ile-Ife, Nigeria. It isolated and
identified the bacterial contaminants in the water
samples. It also evaluated the total coliforms and the total
heterotrophic bacteria in the water samples and
determined the susceptibility of the isolates to
conventional antibiotics. These were with a view to
providing information on the quality of water from
different water sources in the study area. Total bacterial
(TBC) and total coliform counts (TCC) were assessed by
pour plate technique on nutrient and MacConkey agar
plates, respectively, at 37°C for 24 h. Preliminary
identification of bacterial isolates was based on cultural
and morphological characteristics. Identity of isolates was
confirmed using conventional biochemical tests.
Antibiotic susceptibility testing of isolates was done on
Mueller-Hinton agar plates using Kirby-Bauer’s disk
diffusion techniques after incubation at 37°C for 24 h.
Keywords : Antibiotics, Boreholes, Hand-dug Wells.