This study assessed the antibiotic susceptibility pattern of bacterial isolates from selected boreholes and hand-dug wells water in Senior Staff Quarters, Obafemi Awolowo University, Ile-Ife, Nigeria. It isolated and identified the bacterial contaminants in the water samples. It also evaluated the total coliforms and the total heterotrophic bacteria in the water samples and determined the susceptibility of the isolates to conventional antibiotics. These were with a view to providing information on the quality of water from different water sources in the study area. Total bacterial (TBC) and total coliform counts (TCC) were assessed by pour plate technique on nutrient and MacConkey agar plates, respectively, at 37°C for 24 h. Preliminary identification of bacterial isolates was based on cultural and morphological characteristics. Identity of isolates was confirmed using conventional biochemical tests. Antibiotic susceptibility testing of isolates was done on Mueller-Hinton agar plates using Kirby-Bauer’s disk diffusion techniques after incubation at 37°C for 24 h.

Keywords : Antibiotics, Boreholes, Hand-dug Wells.