In the current work, the phytochemical
profile of Euphorbia fischeriana root extract was
characterized on a qualitative and quantitative level, and
then their anticancer activity was proven in human
breast cancer cells by MTT and morphological
evaluation. Qualitative phytochemical analysis showed
that E. fischeriana root extract contains flavonoids,
quinones, terpenoids, triterpenoids, phenolics,
carbohydrates, proteins, tannins, alkaloids, glycosides,
and steroids. A quantity of 1 gm of E. fischeriana root
extract was found to contain 18.29 ± 0.86 mg GAE of
total phenolics and 26.10 ± 1.71 mg QE of total
extract on human breast cancer (MDA-MB-231) was
determined as 104.04 ± 3.27 μg/mL. Micromorphological
analysis revealed that E. fischeriana root extract-treated
human breast cancer (MDA-MB-231) cells demonstrated
cellular membrane breakdown, cellular debris leakage,
and the formation of apoptotic bodies. Thus, E.
fischeriana root extract has potential anticancer action
on human breast cancer (MDA-MB-231) cells. The study
concluded that E. fischeriana root extract could be
highly applicable as an antioxidant and anticancer agent
in the biomedical industry.
Keywords : Euphorbia fischeriana, Phytochemical Profile, Anticancer, Breast Cancer, MDA-MB-231 Cells.