In the current work, the phytochemical profile of Euphorbia fischeriana root extract was characterized on a qualitative and quantitative level, and then their anticancer activity was proven in human breast cancer cells by MTT and morphological evaluation. Qualitative phytochemical analysis showed that E. fischeriana root extract contains flavonoids, quinones, terpenoids, triterpenoids, phenolics, carbohydrates, proteins, tannins, alkaloids, glycosides, and steroids. A quantity of 1 gm of E. fischeriana root extract was found to contain 18.29 ± 0.86 mg GAE of total phenolics and 26.10 ± 1.71 mg QE of total flavonoids.fischeriana root extract on human breast cancer (MDA-MB-231) was determined as 104.04 ± 3.27 μg/mL. Micromorphological analysis revealed that E. fischeriana root extract-treated human breast cancer (MDA-MB-231) cells demonstrated cellular membrane breakdown, cellular debris leakage, and the formation of apoptotic bodies. Thus, E. fischeriana root extract has potential anticancer action on human breast cancer (MDA-MB-231) cells. The study concluded that E. fischeriana root extract could be highly applicable as an antioxidant and anticancer agent in the biomedical industry.

Keywords : Euphorbia fischeriana, Phytochemical Profile, Anticancer, Breast Cancer, MDA-MB-231 Cells.