The effect of Zingiber officinale (Ginger) on two Fungi strains; Aspergilus niger and Rhizopus stolonifer was studied. The plant extracts were obtained by weighing 16g of powdered root of Zingiber officinale and dissolved in 800ml of acid water and distilled water respectively. The extracts were later subjected to gentle concentration using a hot water bath at 800C and then stored in different containers and refrigerated until the extraction was completed. Commercially prepared antiseptic Trichlorophenol (T.C.P) produced by Pfizer Pharmaceutical Company was employed as a standard drug for this study. Various dilutions of the plant extracts were prepared by mixing various volume of the extract and molting fungi growth media at ratio 1/2, 1/5, 1/10, 1/20, 1/50. The two fungi strains tested were freshly isolated from spoiled bread and it was identified in the department of Science Laboratory Technology of Osun State Polytechnic Iree Nigeria. Potatoes dextrose agar was Prepared by suspending 39grames of powdered (P.D.A) in 100ml of distilled water and boiled to dissolve completely,and sterilized with autoclave at 15bar at 1210 c for 15min and various dishes were prepared.The inoculation of fungi was carried out using a standard procedure. All the plates were incubated at 370C for 24hrs, both the acid water and distilled water extract proved to be fungistatic on the two fungi strains tested and standard drug proved to be fungicidal. The (Maximum inhibitory concentration (MIC) of distilled water extract of the plant was observed at 1/30 dilution with (65.5%) mycelia growth inhibition on Aspergillus Rhizopus Stolonifer , while that of acid water extract was observed on Aspergillus niger at 1/10 dilution with (62.0%) mycelia growth inhibition and 1/5 dilution with (55%) mycelia growth inhibition on Rhizopus stolonifer .Based on the result of this study it shows that Zingiber officinale proved to be highly fungistatic when both distilled water and acid water were used as solvents for extraction when compared with the standard drug. There is need for Pharmaceutical companies in Nigeria to exploit the medicinal potential of this plant root in other to serve as source of raw material for the production of standard antifungal drugs.
Keywords : Zingiber officinale, Aspergillus niger, Rhizopus stolonifer , Trichlorophenol, Distilled water, Acid water, Antifungal drug.