Antioxidant Activity, Theaflavin, Total Polyphenol, and Catechin Composition of Camellia sinensis Processing Effluents from Various Factories in Kenya


Authors : Thaddeus Mangenya; Daniel Kariuki; Johnson Kinyua; Martin Obanda; Simon Ochanda; Gervason Moriasi

Volume/Issue : Volume 9 - 2024, Issue 3 - March


Google Scholar : https://tinyurl.com/mt5r43e5

Scribd : https://tinyurl.com/mprzcr66

DOI : https://doi.org/10.38124/ijisrt/IJISRT24MAR1458

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.


Abstract : Research into the antioxidant efficacy and sustainability implications of tea waste, a by-product of the rapidly growing global beverage industry, is increasingly necessary. This study scrutinized samples taken from various factories to explore their capacity for free radical scavenging and potential public health and environmental benefits. Analysis of the samples and reference antioxidant (BHT) revealed a notable dose- dependent rise in free-radical-scavenging action, implying a positive concentration-dependent antioxidant efficacy. Variations in antioxidant activity occurred from tea wastes sourced at various factories, alluding to geography having a pivotal effect on the biological contents and antioxidative capacity. Of particular interest were Boito's and Chelal's cyclone fluff samples that evinced relatively lower potency than those from other factories. Tombe factory's cyclone sample demonstrated a superior capacity for scavenging free radicals at different concentrations, suggesting that particular production or cultivation processes augmented its effectiveness. Notably, some tea waste samples even equalled the potency of BHT (a synthetic antioxidant) when augured to its highest level. Furthermore, this study revealed disparities in Theaflavin and Thearubigin content due to factors like maturation period and processing methods guiding these concentrations. We identified tea waste samples possessing remarkable polyphenol concentration, thereby providing valuable insights for consumers and industries. Further investigations on the chemical composition of tea waste phytocompounds are paramount due to their acclaimed health-promoting properties and the factors influencing their concentrations and antioxidant efficacy.

Keywords : Thearubigin; Catechin; Theaflavin, Polyphenol, Free Radicals.

References :

  1. Chen, Y. Q., Li, Y. F., Shen, C. W. & Xiao, L. Z. Topics and trends in fresh tea (Camellia sinensis) leaf research: A comprehensive bibliometric study. Frontiers in Plant Science vol. 14 Preprint at https://doi.org/10.3389/fpls.2023.1092511 (2023).
  2. Thiruvengadam, V., Binti Baharuddin, N. H. & Jeng Shiun, L. Implementation of life cycle analysis on green tea process. Heliyon 9, (2023).
  3. Atiqah, A., Ansari, M. N. M., Keresahnia, R., Alkhadher, S. A. A. & Al-Amin, A. Q. Recycling and sustainable environmental practices of household tea waste. International Journal of Environmental Technology and Management 22, 352–363 (2019).
  4. Rkmds, H., Mbdk, S., Bmpdkr, S. & Bmpn, B. Environmental Pollution by Tea Processing. JOURNAL OF RESEARCH TECHNOLOGY AND ENGINEERING 1, (2020).
  5. Gao, P., Ogata, Y., Liu, J. & Song, C. The Methods of Tea Waste Reutilization and Economic Benefits Analysis. in 5th International Conference on Mechatronics, Materials, Chemistry and Computer Engineering (ICMMCCE 2017) 418–423 (2017).
  6. Nirmal Halder. Characterization of Tea waste and Cooked waste as a potential feedstock for Biogas production. International journal of research and engineering 3, 11–16 (2016).
  7. Debnath, B., Haldar, D. & Purkait, M. K. Potential and sustainable utilization of tea waste: A review on present status and future trends. Journal of Environmental Chemical Engineering vol. 9 Preprint at https://doi.org/10.1016/j.jece.2021.106179 (2021).
  8. Hrelia, S., Angeloni, C. & Barbalace, M. C. Agri-Food Wastes as Natural Source of Bioactive Antioxidants. Antioxidants vol. 12 Preprint at https://doi.org/10.3390/antiox12020351 (2023).
  9. Rațu, R. N. et al. Application of Agri-Food By-Products in the Food Industry. Agriculture (Switzerland) vol. 13 Preprint at https://doi.org/10.3390/agriculture13081559 (2023).
  10. Yan, Z., Zhong, Y., Duan, Y., Chen, Q. & Li, F. Antioxidant mechanism of tea polyphenols and its impact on health benefits. Animal Nutrition vol. 6 115–123 Preprint at https://doi.org/10.1016/j.aninu.2020.01.001 (2020).
  11. Shaukat, H. et al. Tea polyphenols: extraction techniques and its potency as a nutraceutical. Frontiers in Sustainable Food Systems vol. 7 Preprint at https://doi.org/10.3389/fsufs.2023.1175893 (2023).
  12. Khan, N. & Mukhtar, H. Tea polyphenols in promotion of human health. Nutrients vol. 11 Preprint at https://doi.org/10.3390/nu11010039 (2019).
  13. Khairudin, M. A. S., Jalil, A. M. M. & Hussin, N. Effects of polyphenols in tea (camellia sinensis sp.) on the modulation of gut microbiota in human trials and animal studies. Gastroenterology Insights vol. 12 202–216 Preprint at https://doi.org/10.3390/gastroent12020018 (2021).
  14. Abraham, A. M., Alnemari, R. M., Brüßler, J. & Keck, C. M. Improved antioxidant capacity of black tea waste utilizing plantcrystals. Molecules 26, (2021).
  15. Tong, T., Liu, Y. J., Kang, J., Zhang, C. M. & Kang, S. G. Antioxidant activity and main chemical components of a novel fermented tea. Molecules 24, (2019).
  16. Güçlü Üstündağ, Ö. et al. Black tea processing waste as a source of antioxidant and antimicrobial phenolic compounds. European Food Research and Technology 242, 1523–1532 (2016).
  17. Kotha, R. R., Tareq, F. S., Yildiz, E. & Luthria, D. L. Oxidative Stress and Antioxidants—A Critical Review on In Vitro Antioxidant Assays. Antioxidants vol. 11 Preprint at https://doi.org/10.3390/antiox11122388 (2022).
  18. Roy, P., Mohanty, A. K., Dick, P. & Misra, M. A Review on the Challenges and Choices for Food Waste Valorization: Environmental and Economic Impacts. ACS Environmental Au vol. 3 58–75 Preprint at https://doi.org/10.1021/acsenvironau. 2c00050 (2023).
  19. Augustyniak, A. et al. Natural and synthetic antioxidants: An updated overview. Free Radic Res 44, 1216–1262 (2010).
  20. Xu, X. et al. Synthetic phenolic antioxidants: Metabolism, hazards and mechanism of action. Food Chem 353, (2021).
  21. Kahl, R. & Kappus, H. Toxikologie der synthetischen Antioxidantien BHA und BHT im Vergleich mit dem natürlichen Antioxidans Vitamin E. Z Lebensm Unters Forsch 196, 329–338 (1993).
  22. Brand-Williams, W; Cuvelier, M. E. & Berset, C. Use of a Free Radical Method to Evaluate Antioxidant Activity. 30, 25–30 (1995).
  23. Moriasi, G., Ireri, A. & Ngugi, M. P. In vitro antioxidant activities of the aqueous and methanolic stem bark extracts of Piliostigma thonningii (Schum.). J Evid Based Integr Med 25, 2515690X20937988 (2020).
  24. Spiro, M. & Price, W. E. Determination of Theaflavins in Tea Solution Using the Flavognost Complexation Method. ANALYST vol. 111 (1986).
  25. Menet, M. C., Sang, S., Yang, C. S., Ho, C. T. & Rosen, R. T. Analysis of Theaflavins and Thearubigins from Black Tea Extract by MALDI-TOF Mass Spectrometry. J Agric Food Chem 52, 2455–2461 (2004).
  26. Ahmad, R. S. et al. Quantitative and qualitative portrait of green tea catechins (Gtc) through Hplc. Int J Food Prop 17, 1626–1636 (2014).
  27. Karori SM, Wachira FN, Ngure RM & Mireji PO. Polyphenolic composition and antioxidant activity of Kenyan Tea cultivars. J Pharmacogn Phytochem 3, (2014).
  28. Pradhan, S. & Dubey, R. C. Proximate Analysis, Total Phenolic Content, Antioxidant Activity and Anti-Diabetic Property of Camellia Sinensis and Camellia Assamica. Biochem Cell Arch 20, 15–23 (2020).
  29. Paiva, L., Lima, E., Motta, M., Marcone, M. & Baptista, J. Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis. Current Research in Food Science vol. 3 227–234 Preprint at https://doi.org/10.1016/j.crfs.2020.07.004 (2020).
  30. Ding, J. et al. Tea processing steps affect chemical compositions, enzyme activities, and antioxidant and anti-inflammatory activities of coffee leaves. Food Front 3, 505–516 (2022).
  31. Jayanthy A, Kumar, P. U. & Remashree, A. B. Seasonal and Geographical Variations in Cellular Characters and Chemical Contents in Desmodium gangeticum (L.) DC.-An Ayurvedic Medicinal Plant. ~ 34 ~ International Journal of Herbal Medicine 1, 34–37 (2013).
  32. Fu, C., Yu, P., Wang, M. & Qiu, F. Phytochemical analysis and geographic assessment of flavonoids, coumarins and sesquiterpenes in Artemisia annua L. based on HPLC-DAD quantification and LC-ESI-QTOF-MS/MS confirmation. Food Chem 312, (2020).
  33. Koch, W. et al. Green tea quality evaluation based on its catechins and metals composition in combination with chemometric analysis. Molecules 23, (2018).
  34. Korkina, L. G. Phenylpropanoids as naturally occurring antioxidants: From plant defense to human health. Cell Mol Biol 53, 15–25 (2007).
  35. Caleja, C., Barros, L., Antonio, A. L., Oliveira, M. B. P. P. & Ferreira, I. C. F. R. A comparative study between natural and synthetic antioxidants: Evaluation of their performance after incorporation into biscuits. Food Chem 216, 342–346 (2017).
  36. Lourenço, S. C., Moldão-Martins, M. & Alves, V. D. Antioxidants of natural plant origins: From sources to food industry applications. Molecules 24, 14–16 (2019).
  37. Moriasi, G. A., Ireri, A. M., Nelson, E. M. & Ngugi, M. P. In vivo anti-inflammatory, anti-nociceptive, and in vitro antioxidant efficacy, and acute oral toxicity effects of the aqueous and methanolic stem bark extracts of Lonchocarpus eriocalyx (Harms.). Heliyon 7, e07145 (2021).
  38. Paiva, L., Rego, C., Lima, E., Marcone, M. & Baptista, J. Comparative analysis of the polyphenols, caffeine, and antioxidant activities of green tea, white tea, and flowers from azorean camellia sinensis varieties affected by different harvested and processing conditions. Antioxidants 10, 1–16 (2021).
  39. Liu, W. et al. Influence of environmental factors on the active substance production and antioxidant activity in Potentilla fruticosa L. and its quality assessment. Sci Rep 6, (2016).
  40. Yang, J. & Liu, R. H. The phenolic profiles and antioxidant activity in different types of tea. Int J Food Sci Technol 48, 163–171 (2013).
  41. Hossain, M. A. et al. Optimization of the factors affecting BT-2 black tea fermentation by observing their combined effects on the quality parameters of made tea using Response Surface Methodology (RSM). Heliyon 8, (2022).
  42. Mangenya, T. M. ASSESSMENT OF CATECHINS AND OTHER POLYPHENOLS AS PARAMETERS OF TEA QUALITY IN SELECTED ECOLOGICAL ZONES IN KENYA. (Jomo Kenyatta University of Agriculture and Technology, 2016).
  43. Koch, W., Kukula-Koch, W. & Głowniak, K. Catechin composition and antioxidant activity of black teas in relation to brewing time. J AOAC Int 100, 1694–1699 (2017).
  44. Gonçalves Bortolini, D., Windson Isidoro Haminiuk, C., Cristina Pedro, A., de Andrade Arruda Fernandes, I. & Maria Maciel, G. Processing, chemical signature and food industry applications of Camellia sinensis teas: An overview. Food Chem X 12, (2021).
  45. Wong, M., Sirisena, S. & Ng, K. Phytochemical profile of differently processed tea: A review. Journal of Food Science vol. 87 1925–1942 Preprint at https://doi.org/10.1111/1750-3841.16137 (2022).
  46. Sanlier, N., Atik, İ. & Atik, A. A minireview of effects of white tea consumption on diseases. Trends in Food Science and Technology vol. 82 82–88 Preprint at https://doi.org/10.1016/j.tifs.2018.10.004 (2018).
  47. Korani, M. S., Farbood, Y., Sarkaki, A., Fathi Moghaddam, H. & Taghi Mansouri, M. Protective effects of gallic acid against chronic cerebral hypoperfusion-induced cognitive deficit and brain oxidative damage in rats. Eur J Pharmacol 733, 62–67 (2014).
  48. Ojo, O. A., Rotimi, D. E., Ojo, A. B., Ogunlakin, A. D. & Ajiboye, B. O. Gallic acid abates cadmium chloride toxicity via alteration of neurotransmitters and modulation of inflammatory markers in Wistar rats. Sci Rep 13, (2023).
  49. Farhan, M. Green Tea Catechins: Nature’s Way of Preventing and Treating Cancer. International Journal of Molecular Sciences vol. 23 Preprint at https://doi.org/10.3390/ijms231810713 (2022).
  50. Rodak, K., Kokot, I. & Kratz, E. M. Caffeine as a factor influencing the functioning of the human body—friend or foe? Nutrients vol. 13 Preprint at https://doi.org/10.3390/nu13093088 (2021).
  51. Seo, H. et al. (−)-epicatechin-enriched extract from camellia sinensis improves regulation of muscle mass and function: Results from a randomized controlled trial. Antioxidants 10, (2021).
  52. Zhao, T., Li, C., Wang, S. & Song, X. Green Tea (Camellia sinensis): A Review of Its Phytochemistry, Pharmacology, and Toxicology. Molecules vol. 27 Preprint at https://doi.org/10.3390/molecules27123909 (2022).
  53. Abbas, S. & Wink, M. Epigallocatechin gallate from green tea (Camellia sinensis) increases lifespan and stress resistance in Caenorhabditis elegans. Planta Med 75, 216–221 (2009).
  54. Prasanth, M. I., Sivamaruthi, B. S., Chaiyasut, C. & Tencomnao, T. A review of the role of green tea (camellia sinensis) in antiphotoaging, stress resistance, neuroprotection, and autophagy. Nutrients vol. 11 Preprint at https://doi.org/10.3390/nu11020474 (2019).
  55. Yang, Z. A., Xu, Y. A., Jie, G. A., He, P. & Tu, Y. Study on the antioxidant activity of tea flowers (Camellia sinensis). Asia Pac J Clin Nutr vol. 16 (2007).

Research into the antioxidant efficacy and sustainability implications of tea waste, a by-product of the rapidly growing global beverage industry, is increasingly necessary. This study scrutinized samples taken from various factories to explore their capacity for free radical scavenging and potential public health and environmental benefits. Analysis of the samples and reference antioxidant (BHT) revealed a notable dose- dependent rise in free-radical-scavenging action, implying a positive concentration-dependent antioxidant efficacy. Variations in antioxidant activity occurred from tea wastes sourced at various factories, alluding to geography having a pivotal effect on the biological contents and antioxidative capacity. Of particular interest were Boito's and Chelal's cyclone fluff samples that evinced relatively lower potency than those from other factories. Tombe factory's cyclone sample demonstrated a superior capacity for scavenging free radicals at different concentrations, suggesting that particular production or cultivation processes augmented its effectiveness. Notably, some tea waste samples even equalled the potency of BHT (a synthetic antioxidant) when augured to its highest level. Furthermore, this study revealed disparities in Theaflavin and Thearubigin content due to factors like maturation period and processing methods guiding these concentrations. We identified tea waste samples possessing remarkable polyphenol concentration, thereby providing valuable insights for consumers and industries. Further investigations on the chemical composition of tea waste phytocompounds are paramount due to their acclaimed health-promoting properties and the factors influencing their concentrations and antioxidant efficacy.

Keywords : Thearubigin; Catechin; Theaflavin, Polyphenol, Free Radicals.

Never miss an update from Papermashup

Get notified about the latest tutorials and downloads.

Subscribe by Email

Get alerts directly into your inbox after each post and stay updated.
Subscribe
OR

Subscribe by RSS

Add our RSS to your feedreader to get regular updates from us.
Subscribe