Antipyretic and Analgesic Activity of Dioscorea bulbifera on Wistar Albino Rats – Three Arm Randomized Control Trial


Authors : A. S. R. Perera; N. Varnakulendran

Volume/Issue : Volume 11 - 2026, Issue 1 - January

Google Scholar : https://tinyurl.com/bdhf7z69

Scribd : https://tinyurl.com/2mc7m2a4

DOI : https://doi.org/10.38124/ijisrt/26jan1327

PlumX Metrics

Semantic Scholar

ResearchGate

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Fever, or pyrexia, is defined as an elevation in body temperature beyond normal physiological limits and pain an unpleasant sensory and emotional experience occur in some types of fever. Dioscorea bulbifera leaves are traditionally used for managing fever with aches and pain as per Siddha Literature. Based on these traditional claims, the present study aimed to scientifically evaluate the antipyretic and analgesic activities of Diacorea bulbifera leaf decoction using experimental animal models. For antipyretic activity, 18 male Wistar albino rats were divided into three groups: control, standard, and test. Fever was induced using 15% w/v Brewer's yeast administered subcutaneously. After 18 hours, rectal temperatures were recorded at 30, 60, 90, and 120 minutes following treatment. The control group received 1 ml/kg normal saline, the standard group received paracetamol syrup, and the test group received Diacorea bulbifera leaf decoction according to standard dose calculation. For analgesic activity, 18 female Wistar albino rats were divided similarly, and the tail immersion method was used. Tail withdrawal times were recorded at 30, 60, 90, and 120 minutes after administering saline, diclofenac and diascorea bulbifera decoction. The Dioscorea bulbifera decoction significantly reduced fever, showing effects comparable to paracetamol after 90 minutes. It also exhibited notable analgesic activity from the 60th minute onward, with stronger and longer-lasting effects than diclofenac. Dioscorea bulbifera leaf decoction demonstrates significant antipyretic and analgesic effects, supporting its traditional use in treating fever and pain.

Keywords : Antipyretic, Analgesic, Decoction, Wistar Albino Rats, Rectal Temperature.

References :

  1. Kuppusami Mudheliyar, K. N. (2012), siddha medicine general Page No - 319-394.
  2. Kumar P, Clark M, eds. Kumar & Clark's Clinical Medicine. 6th ed. Elsevier; 2006.
  3. Ralston S, Penman I, Strachan M, Hobson R, eds. Davidson's Principles and Practice of Medicine. 23rd ed. Elsevier; 2015.
  4. Verma, Avinash & Vimal, Singh, Kumar & Gupta. (2024). Pharmacological, and Phytochemical Profile of Dioscorea bulbifera (Dioscoreaceae): A Review.
  5. Singh S, Sangraula H, Singh PK, Sarraf DP. Evaluation of Antinociceptive Activity of Ficus Religiosa Root Extract in Swiss Albino Mice. Kathmandu Univ Med J. 2022;80(4):412-6.
  6. Mishra A. Chemical constituents and medicinal properties of Dioscorea bulbifera. International Journal of Pharmaceutical Sciences and Research. 2013;4(5):1570-1582.
  7. Verma N, Jha KK, Kumar U, Deepak K, Singh NK, Singh AK, Sharma R. Biological properties, phytochemistry and traditional uses of Mahua (Madhuca longifolia): A Review. International Journal of Advance Research and Innovation 2014; 2(3): 630-8.
  8. Soni, anshita. (2011). Evaluation of Pharmacological Potentials of Methanolic leaf Extract of Dioscorea bulbifera (Sopteacae) against Pyrexia. Journal of Pharmacy Research. 4. 2011.
  9. Singh S, Sangraula H, Singh PK, Sarraf DP. Evaluation of Antinociceptive Activity of Ficus Religiosa Root Extract in Swiss Albino Mice. Kathmandu Univ Med J. 2022;80(4):412-6.
  10. Shekhawat, N. S., Sharma, K., & Sharma, S. (2010). Anti-inflammatory, analgesic, and antipyretic activities of Dioscorea bulbifera (Koenig) J.F. Macbride in experimental models. International Journal of Pharma and Bio Sciences, 1(3), 122-130
  11. Sangeetha, K. N., Sujatha, S., & Muthusamy, K. (2016). Phytochemical analysis and pharmacological evaluation of Dioscorea bulbifera for anti-inflammatory and antioxidant activities. International Journal of Pharmaceutical Sciences and Research, 7(3), 1107-1115.
  12. Salau, B. A., Osungbaro, J. O., & Ajibola, V. O. (2013). In vitro assessment of the toxicological effects of Dioscorea bulbifera on human cells. Journal of Toxicology and Environmental Health Sciences, 5(1), 12-20.
  13. Murugesamuthalıyar. (2008), Medicinal plants division In Siddha material medica, 2 ed,Chennal indian maruthavam homeopathy page no 218.
  14. Kuppusami Mudheliyar KN. Siddha medicine general. 2012:319-394.
  15. Piratheepkumar R, Vijitha P. Evaluation of anti-pyretic activity of Plectranthus vettiveroides. Int J Complement Alt Med. 2021;14(3):107-111.
  16. Ogoina D. Remittent fever: An overview of diagnosis and management. Journal of Medicine and Medical Sciences. 2011;2(10):1234-1241.
  17. Flecknell PA. The relief of pain in laboratory animals. Laboratory Animals. 1984;18:147-160.
  18. Sangeetha KN, Sujatha S, Muthusamy K. Phytochemical analysis and pharmacological evaluation of Dioscorea bulbifera for anti-inflammatory and antioxidant activities. International Journal of Pharmaceutical Sciences and Research. 2016;7(3):1107-1115.
  19. Shekhawat NS, Sharma K, Sharma S. Anti-inflammatory, analgesic, and antipyretic activities of Dioscorea bulbifera (Koenig) J.F. Macbride in experimental models. International Journal of Pharma and Bio Sciences. 2010;1(3):122-130.
  20. Soni A. Evaluation of Pharmacological Potentials of Methanolic leaf Extract of Dioscorea bulbifera (Sopteacae) against Pyrexia. Journal of Pharmacy Research. 2011;4:2011.
  21. Kumar A, Singh UP. International Journal of Pharmaceutical Sciences and Medicine. 2024;9:1-8.
  22. Kushwaha, Archana & Mishra, Tulika. (2020). A Brief Review On Dioscorea Bulbifera Linn: Constituents And Pharmacological Effects. European Journal Of Pharmaceutical And Medical Research. 7. 320-327.
  23. Ghlichloo I, Gerriets V. Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) [Updated 2023 May 1]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK547742/
  24. Patel DM, Galani VJ. Evaluation of neuropharmacological activity of dioscorea bulbifera using various experimental models. Adv Plants Agric Res. 2017;7(1):214-219. DOI: 10.15406/apar.2017.07.00241
  25. Ahmad, Mahmood & Iqbal, Muhammad & Murtaza, Ghulam. (2009). Comparison of bioavailability and pharmacokinetics of diclofenac sodium and diclofenac potassium in normal and dehydrated rabbits. Yao xue xue bao, Acta pharmaceutica Sinica. 44. 80-4.

Fever, or pyrexia, is defined as an elevation in body temperature beyond normal physiological limits and pain an unpleasant sensory and emotional experience occur in some types of fever. Dioscorea bulbifera leaves are traditionally used for managing fever with aches and pain as per Siddha Literature. Based on these traditional claims, the present study aimed to scientifically evaluate the antipyretic and analgesic activities of Diacorea bulbifera leaf decoction using experimental animal models. For antipyretic activity, 18 male Wistar albino rats were divided into three groups: control, standard, and test. Fever was induced using 15% w/v Brewer's yeast administered subcutaneously. After 18 hours, rectal temperatures were recorded at 30, 60, 90, and 120 minutes following treatment. The control group received 1 ml/kg normal saline, the standard group received paracetamol syrup, and the test group received Diacorea bulbifera leaf decoction according to standard dose calculation. For analgesic activity, 18 female Wistar albino rats were divided similarly, and the tail immersion method was used. Tail withdrawal times were recorded at 30, 60, 90, and 120 minutes after administering saline, diclofenac and diascorea bulbifera decoction. The Dioscorea bulbifera decoction significantly reduced fever, showing effects comparable to paracetamol after 90 minutes. It also exhibited notable analgesic activity from the 60th minute onward, with stronger and longer-lasting effects than diclofenac. Dioscorea bulbifera leaf decoction demonstrates significant antipyretic and analgesic effects, supporting its traditional use in treating fever and pain.

Keywords : Antipyretic, Analgesic, Decoction, Wistar Albino Rats, Rectal Temperature.

Paper Submission Last Date
28 - February - 2026

SUBMIT YOUR PAPER CALL FOR PAPERS
Video Explanation for Published paper

Never miss an update from Papermashup

Get notified about the latest tutorials and downloads.

Subscribe by Email

Get alerts directly into your inbox after each post and stay updated.
Subscribe
OR

Subscribe by RSS

Add our RSS to your feedreader to get regular updates from us.
Subscribe