Authors :
Abiodun,Moses Oluwaseun; Ekesiobi, Anthony Obinna; Abiodun Iyabode Khadijat; Onyenweife, Leonard Chikwado
Volume/Issue :
Volume 6 - 2021, Issue 11 - November
Google Scholar :
http://bitly.ws/gu88
Scribd :
https://bit.ly/3xMpeas
Abstract :
This study investigated trypanosomal activity,
haematological and lipid profile effects of Garcinia kola
aqueous seed extract and Berenil drug in typanosomiasis
induced Wistar rat. The extract was obtained through
Maceration method with distilled water, In vitro activity of
the extract and Berenil drug was determined using rapid
matching techniques at 3 hours post incubation period
with different doses of the extract and 3.5mg of Berenil
drug. In vivo study was done using 62 Wister rats, divided
into 10 groups. Group A (normal control): non infected
and non treated, Group (B-H) were infected by intra
peritoneal inoculation of 1mL of parasitized blood
containing 1.0105
/ml of T. brucei gambianse parasites and
treated with different doses of Garcinia kola seed extract,
200 – 1400mg, Group I: infected and treated with standard
drug dose, Group J: infected and non treated (Pathological
control). The treatment lasted for 21 days at 3 days
interval. Hematological and lipid profile effects were
determined by colorimetric method. Data were analyzed
statistically using Anova at 0.01 significant differences.
Various hematological and lipid profile effects were
observed in all the extract treated and control groups.
Garcinia kola seed extract exhibited trypanosostatic at dose
of <400mg and trypanocidal activity at doses > 400mg in
both in vitro and in vivo, compared with trypanocidal
activity of standard Berenil drug dose. Garcinia kola seed
extract produced moderate side effect on haematological
and lipids profile parameters of wistar rat at doses
<400mg in the extract treated groups when compared with
Berenil drug which produced higher toxicity effect on
heamatological and lipid profile parameters at standard
dose. This study revealed that Garcinia kola aqueous seed
extract may be used to design drug for the treatment of
trypanosomiasis at lower concentration therefore, the use
of Berenil drug in human and animal treatment should be
discontinued
Keywords :
Trypanosomiasis, Trypanosostatic, Wister rat, Berenil, Garciniakola.
This study investigated trypanosomal activity,
haematological and lipid profile effects of Garcinia kola
aqueous seed extract and Berenil drug in typanosomiasis
induced Wistar rat. The extract was obtained through
Maceration method with distilled water, In vitro activity of
the extract and Berenil drug was determined using rapid
matching techniques at 3 hours post incubation period
with different doses of the extract and 3.5mg of Berenil
drug. In vivo study was done using 62 Wister rats, divided
into 10 groups. Group A (normal control): non infected
and non treated, Group (B-H) were infected by intra
peritoneal inoculation of 1mL of parasitized blood
containing 1.0105
/ml of T. brucei gambianse parasites and
treated with different doses of Garcinia kola seed extract,
200 – 1400mg, Group I: infected and treated with standard
drug dose, Group J: infected and non treated (Pathological
control). The treatment lasted for 21 days at 3 days
interval. Hematological and lipid profile effects were
determined by colorimetric method. Data were analyzed
statistically using Anova at 0.01 significant differences.
Various hematological and lipid profile effects were
observed in all the extract treated and control groups.
Garcinia kola seed extract exhibited trypanosostatic at dose
of <400mg and trypanocidal activity at doses > 400mg in
both in vitro and in vivo, compared with trypanocidal
activity of standard Berenil drug dose. Garcinia kola seed
extract produced moderate side effect on haematological
and lipids profile parameters of wistar rat at doses
<400mg in the extract treated groups when compared with
Berenil drug which produced higher toxicity effect on
heamatological and lipid profile parameters at standard
dose. This study revealed that Garcinia kola aqueous seed
extract may be used to design drug for the treatment of
trypanosomiasis at lower concentration therefore, the use
of Berenil drug in human and animal treatment should be
discontinued
Keywords :
Trypanosomiasis, Trypanosostatic, Wister rat, Berenil, Garciniakola.
