The first instance of human immunodeficiency virus reported in 1984 and HIV/AIDS declared as public health emergency in 2002 in Ethiopia. Human immunodeficiency virus is a chronic infection caused by type 1 and type 2 of human immunodeficiency virus, which infects human only. The major transmission is sexual contact but others can also include transmission through occupational and non- occupational exposures. Work-related revelation is when someone work in health care setting is possibly exposed to Human immunodeficiency virus, because of blood and body fluid contacts. The main objective of this paper is to assess knowledge, attitude, and practice of health workers (HCWs) about post exposure prophylaxis (PEP) for Human immunodeficiency virus (HIV) among health care workers in Mertule mariam Hospital and health center. METHODS: Institutional based cross-sectional study design was carried out from January 01 to May 30,2021. A total of 124 health care workers were take part in the study health facilities. Data were collected using pretested self- administered questionnaire and analyzed using SPSS in version 21. RESULTS: Among the total participants, 73% were males and most of them were between 20 to 30 years’ age range. Around 59.9% of them were single. Ninety-twopoint seven percent participants have heard about post exposure prophylaxis, 52.8% had unfavorable attitude towards post exposure prophylaxis and 7.2% had poor knowledge towards post exposure prophylaxis for human immunodeficiency virus. CONCLUSION: Despite there were adequate knowledge towards post exposure prophylaxis, participants’ attitude and practice of PEP is low and intent of initiation was delayed for PEP.

Keywords : Knowledge, attitude, practice, PEP, HIV, health workers.