Scientists have been operating biomedical research by relying on flat monolayer 2D cell culture for a very long time. The more innovative the researches have become, the more efficiently the diseases can be cured, and inevitably higher the demand in research methods and tools. In our modern era, cancer is undeniably a threat in society and understanding the intricate interplay of tumor cell heterogeneity and plasticity holds the key to cure cancer. Since 2D models are too plain, especially in complicated studies like cancer, applying 2D cell-culture to study cancer research can be a huge obstacle. In order to decode the dynamic response of tumor cells to their microenvironment, a more physiologically relevant environment for studying cell-cell interaction and drug responses is required. Thus, 3D cell culture method is introduced to optimize the research requirement. In this paper we present a 3D microfluidic device integrated with impedance micro- sensing which is an advanced fusion of microfluidic technology with a 3D cell culture method and micro- sensing. Additionally, 3D cell culture methods as presented in this paper prove to be a better technique to obtain live cells in viability assays than 2D cell cultures. Through this paper, we hope to present an enhancing technology and showcase how 3D cell cultures are the next big thing in tumor cell research and the treatment of cancer.

Keywords : 2D Cell Cultures; 3D Cell Cultures; Microfluidic Devices; Impedance Micro-Sensing; MCF- 7(Breast Cancer Cell).