Authors :
Rumman Jan; Pratyush Amatya; Min Thant Nuang; Maneesh Reddy
Volume/Issue :
Volume 8 - 2023, Issue 10 - October
Google Scholar :
https://tinyurl.com/32cj9ckw
Scribd :
https://tinyurl.com/msfffden
DOI :
https://doi.org/10.5281/zenodo.10099226
Abstract :
Scientists have been operating biomedical
research by relying on flat monolayer 2D cell culture for
a very long time. The more innovative the researches
have become, the more efficiently the diseases can be
cured, and inevitably higher the demand in research
methods and tools. In our modern era, cancer is
undeniably a threat in society and understanding the
intricate interplay of tumor cell heterogeneity and
plasticity holds the key to cure cancer. Since 2D models
are too plain, especially in complicated studies like
cancer, applying 2D cell-culture to study cancer research
can be a huge obstacle. In order to decode the dynamic
response of tumor cells to their microenvironment, a
more physiologically relevant environment for studying
cell-cell interaction and drug responses is required.
Thus, 3D cell culture method is introduced to optimize
the research requirement. In this paper we present a 3D
microfluidic device integrated with impedance micro-
sensing which is an advanced fusion of microfluidic
technology with a 3D cell culture method and micro-
sensing. Additionally, 3D cell culture methods as
presented in this paper prove to be a better technique to
obtain live cells in viability assays than 2D cell cultures.
Through this paper, we hope to present an enhancing
technology and showcase how 3D cell cultures are the
next big thing in tumor cell research and the treatment
of cancer.
Keywords :
2D Cell Cultures; 3D Cell Cultures; Microfluidic Devices; Impedance Micro-Sensing; MCF- 7(Breast Cancer Cell).
Scientists have been operating biomedical
research by relying on flat monolayer 2D cell culture for
a very long time. The more innovative the researches
have become, the more efficiently the diseases can be
cured, and inevitably higher the demand in research
methods and tools. In our modern era, cancer is
undeniably a threat in society and understanding the
intricate interplay of tumor cell heterogeneity and
plasticity holds the key to cure cancer. Since 2D models
are too plain, especially in complicated studies like
cancer, applying 2D cell-culture to study cancer research
can be a huge obstacle. In order to decode the dynamic
response of tumor cells to their microenvironment, a
more physiologically relevant environment for studying
cell-cell interaction and drug responses is required.
Thus, 3D cell culture method is introduced to optimize
the research requirement. In this paper we present a 3D
microfluidic device integrated with impedance micro-
sensing which is an advanced fusion of microfluidic
technology with a 3D cell culture method and micro-
sensing. Additionally, 3D cell culture methods as
presented in this paper prove to be a better technique to
obtain live cells in viability assays than 2D cell cultures.
Through this paper, we hope to present an enhancing
technology and showcase how 3D cell cultures are the
next big thing in tumor cell research and the treatment
of cancer.
Keywords :
2D Cell Cultures; 3D Cell Cultures; Microfluidic Devices; Impedance Micro-Sensing; MCF- 7(Breast Cancer Cell).
