Benzyl Isothiocyanate Loaded Gelatin Nanoparticles Display Unique in Vitro Antioxidant Prospects


Authors : Ahana Sinha; Gouri Karan; Mrinmoyee Modak; Piyali Bar; Kaustav Tripathy; Dibyendu Giri; Debjani Chatterjee; Anirban Majumder; Angsuman Das Chaudhuri; Aranya Ghosh; Rimpa Malakar; Sujata Maiti Choudhury

Volume/Issue : Volume 9 - 2024, Issue 9 - September

Google Scholar : https://tinyurl.com/4m9fdyk7

Scribd : https://tinyurl.com/48a65h6x

DOI : https://doi.org/10.38124/ijisrt/IJISRT24SEP1449

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Abstract : This present investigation focuses on the fact that nanoformulation of phytochemicals could enhance the therapeutic capacity in different physiological systems by enhancing hydrophilicity and bioavailability. In this study gelatin nano-formulation of benzyl isothiocyanate (BITC) was prepared and characterized by dynamic light scattering and UV-Visible spectrometry. Then antioxidant activity of BITC and BITC-gelatin NPs was determined in different concentrations through measuring 2,2- diphenyl-1- picrylhydrazyl (DPPH), superoxide, hydroxyl radical, nitric oxide scavenging and lipid peroxidation inhibition activities. DLS and UV-Vis study revealed the production of uniform nanosized particles and effective encapsulation of BITC respectively. The results of antioxidant assays suggested that BITC-gelatin NPs more effectively scavenged free radicals and inhibited lipid peroxidation compared to free BITC. The findings proposed that gelatin formulated BITC nanoparticles could be effective against oxidative stress related disorders.

Keywords : Benzyl Isothiocyanate, Gelatin Nanoparticles, Oxidative Stress, Antioxidant Activity, Lipid Peroxidation Inhibition.

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This present investigation focuses on the fact that nanoformulation of phytochemicals could enhance the therapeutic capacity in different physiological systems by enhancing hydrophilicity and bioavailability. In this study gelatin nano-formulation of benzyl isothiocyanate (BITC) was prepared and characterized by dynamic light scattering and UV-Visible spectrometry. Then antioxidant activity of BITC and BITC-gelatin NPs was determined in different concentrations through measuring 2,2- diphenyl-1- picrylhydrazyl (DPPH), superoxide, hydroxyl radical, nitric oxide scavenging and lipid peroxidation inhibition activities. DLS and UV-Vis study revealed the production of uniform nanosized particles and effective encapsulation of BITC respectively. The results of antioxidant assays suggested that BITC-gelatin NPs more effectively scavenged free radicals and inhibited lipid peroxidation compared to free BITC. The findings proposed that gelatin formulated BITC nanoparticles could be effective against oxidative stress related disorders.

Keywords : Benzyl Isothiocyanate, Gelatin Nanoparticles, Oxidative Stress, Antioxidant Activity, Lipid Peroxidation Inhibition.

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