Biodiesel is defined as the mono-alkyl esters
of long-chain fatty acids derivative from vegetable oils
or animal fats using a catalyst. Neem oil is non-edible oil
and it is an effective alternative source for the
production of biodiesel. But till now, heterogeneous
chemical catalysts are used to convert the neem oil to
biodiesel. Here, we focused on the higher production of
biodiesel from neem oil using microbial lipase as a
catalyst. Lipase was produced from Pseudomonas
aeruginosa TEN01. The productivity of the organism
was examined for free cell and whole-cell
immobilization methods. Both free and immobilized
cells produced lipase activity was 4.6 U/ml.min-1 on
48h. Produced lipase was partially purified by
ammonium sulphate precipitation and dialysis.
Partially purified lipase-catalyzed the reaction of
methyl ester formation from triglycerides present in the
neem oil. The primary characterization was done by
TLC and the biodiesel was confirmed through flash
point and fire point test.
Keywords : Biodiesel; Neem Oil; Lipase; TLC; Flash Point Test.