Biodiesel Production using Neem Oil with Lipase from Pseudomonas aeruginosa TEN01

Authors : Saravanan R, Tamil Elakkiya V, Sivakumar D.B.

Volume/Issue : Volume 4 - 2019, Issue 7 - July

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Biodiesel is defined as the mono-alkyl esters of long-chain fatty acids derivative from vegetable oils or animal fats using a catalyst. Neem oil is non-edible oil and it is an effective alternative source for the production of biodiesel. But till now, heterogeneous chemical catalysts are used to convert the neem oil to biodiesel. Here, we focused on the higher production of biodiesel from neem oil using microbial lipase as a catalyst. Lipase was produced from Pseudomonas aeruginosa TEN01. The productivity of the organism was examined for free cell and whole-cell immobilization methods. Both free and immobilized cells produced lipase activity was 4.6 U/ml.min-1 on 48h. Produced lipase was partially purified by ammonium sulphate precipitation and dialysis. Partially purified lipase-catalyzed the reaction of methyl ester formation from triglycerides present in the neem oil. The primary characterization was done by TLC and the biodiesel was confirmed through flash point and fire point test.

Keywords : Biodiesel; Neem Oil; Lipase; TLC; Flash Point Test.


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