A biofertilizer is a biological material that contains active or dormant microbe cells (bacteria, actinomycetes, fungi, algae) that help fix atmospheric N or solubilize/mobilize soil nutrients while also secreting growth-promoting chemicals to boost growth and yield. This paper reviews research and development regarding bio-fertilizers in Nepal. The usage of biofertilizers can be very beneficial for maintaining the agricultural systems. A number of research groups and organizations have been working on biofertilizer research and development to improve the usage of biofertilizers in Nepal's agriculture. In Nepal, Azotobacter and Rhizobium strains are used in the production of bio-fertilizers. However, there have been fewer attempts to identify and classify associative N fixers. If Nepal works more on N fixers along with standard production of biofertilizers, it would help in the increment of agricultural yield by improving soil quality.

Keywords : Azotobacter, Rhizobium, Soil, Yield