Authors :
Fathima Shahithaa; Hassanal Harrisb; Mohammed Al-Sibania; Ab. Rahim; Mohd Hasbib; Ahmed Al Harrasic; Asiya Obaid Abdallah Al Saadia
Volume/Issue :
Volume 7 - 2022, Issue 2 - February
Google Scholar :
http://bitly.ws/gu88
Scribd :
https://bit.ly/3tDoZwU
DOI :
https://doi.org/10.5281/zenodo.6337882
Abstract :
Hydroxyapatite (HA) crystals inside the
pores of hydroxyethyl cellulose (HEC) sponges have
been developed using simulated body fluid (SBF) in a
green chemistry approach and has been used as a
scaffold material. Porous HEC sponges were prepared
by freeze-drying method. HEC is a water soluble
polymer which is non-toxic and biocompatible. The
crystallization of hydroxyapatite (HA) was done by
immersing HEC sponges (1 wt%, 3 wt% and 5 wt%)
into various concentration of simulated body fluid (SBF)
for different time periods. For the analysis of these
materials, various bulk and particle level
characterization techniques have been employed, which
includes Field transmission scanning electron
microscopy (FESEM), Scanning Electron Microscope
(SEM), Fourier transform infrared spectroscopy
(FTIR), X-ray diffraction spectroscopy (XRD) and
mechanical testing. Energy dispersive X-ray (EDX)
analysis was used to confirm the deposition of apatite on
the surface of HEC sponges. It was found that porosity,
pore size and pore inter connectivity depends upon the
concentration of the HEC. The size of the HA crystals
increased with the concentration of the HEC. The
sponges had pores with diameter~2-200μm and pores
were interconnected. In-vitro testing with human dental
pulps stem cell (DPSC) was also conducted to assess its
biocompatibility. In-vitro bioactivity and
biodegradability studies showed that the HEC/HA
scaffolds were bioactive as well as bioresorbable.
Combining hydroxyapatite into hydroxyethyl cellulose
may generate a composite with favorable mechanical
and chemical properties that are appropriate for
various medical applications.
Keywords :
Hydroxyapatite, Hydroxyethyl Cellulose, Scaffolds, Sponges, Tissue Engineering.
Hydroxyapatite (HA) crystals inside the
pores of hydroxyethyl cellulose (HEC) sponges have
been developed using simulated body fluid (SBF) in a
green chemistry approach and has been used as a
scaffold material. Porous HEC sponges were prepared
by freeze-drying method. HEC is a water soluble
polymer which is non-toxic and biocompatible. The
crystallization of hydroxyapatite (HA) was done by
immersing HEC sponges (1 wt%, 3 wt% and 5 wt%)
into various concentration of simulated body fluid (SBF)
for different time periods. For the analysis of these
materials, various bulk and particle level
characterization techniques have been employed, which
includes Field transmission scanning electron
microscopy (FESEM), Scanning Electron Microscope
(SEM), Fourier transform infrared spectroscopy
(FTIR), X-ray diffraction spectroscopy (XRD) and
mechanical testing. Energy dispersive X-ray (EDX)
analysis was used to confirm the deposition of apatite on
the surface of HEC sponges. It was found that porosity,
pore size and pore inter connectivity depends upon the
concentration of the HEC. The size of the HA crystals
increased with the concentration of the HEC. The
sponges had pores with diameter~2-200μm and pores
were interconnected. In-vitro testing with human dental
pulps stem cell (DPSC) was also conducted to assess its
biocompatibility. In-vitro bioactivity and
biodegradability studies showed that the HEC/HA
scaffolds were bioactive as well as bioresorbable.
Combining hydroxyapatite into hydroxyethyl cellulose
may generate a composite with favorable mechanical
and chemical properties that are appropriate for
various medical applications.
Keywords :
Hydroxyapatite, Hydroxyethyl Cellulose, Scaffolds, Sponges, Tissue Engineering.
