Authors : Gowthami M.; Sindhushri Chauhan; Shivakantkumar Adhikari

Volume/Issue : Volume 11 - 2026, Issue 8 - August

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Google Scholar : https://tinyurl.com/49y8sukw

DOI : https://doi.org/10.38124/ijisrt/26aug1001

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

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Abstract : Bacillus amyloliquefaciens is a Gram-positive, spore-forming bacterium found in soil, plants, water, and food, known for promoting plant growth by fixing nitrogen, solubilizing phosphate, and producing antimicrobial compounds. In this study, Bacillus amyloliquefaciens PGB240 was used to synthesize silver (Ag), titanium dioxide (TiO₂), and silvertitanium dioxide (Ag-TiO₂) nanoparticles (NPs). Their physicochemical properties were analyzed using UV-Vis, XRD, EDX, SEM, FTIR, and DLS techniques. Antibacterial activity was tested against Streptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumoniae, and Escherichia coli, with all NPs showing significant inhibition.

Keywords : Biosynthesized Nanoparticles, Antibacterial Properties, Antioxidant Activities, Seed Germinations, DPPH Free Radicals, X-ray Diffraction, Dynamic Light Scattering

References :

1. WoldemariamYohannes K, Wan Z, Yu Q, Li H, Wei X, Liu Y, Wang J, Sun B. Prebiotic, probiotic, antimicrobial, and functional food applications of Bacillus amyloliquefaciens. J Agric Food Chem. 2020;68:14709–14727. https://doi.org/10.1021/acs.jafc.0c06396.
2. Algburi A, Alazzawi SA, Al-Ezzy AIA, Weeks R, Chistyakov V, Chikindas ML. Potential probiotics Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 co-aggregate with clinical isolates of Proteus mirabilis and prevent biofilm formation. Probiotics Antimicrob Proteins. 2020;12:1471–1483. https://doi.org/10.1007/s12602-020-09631-0.
3. Du H, Yao W, Kulyar MFEA, Ding Y, Zhu H, Pan H, Li K, Bhutta ZA, Liu S, Li J. Effects of Bacillus amyloliquefaciens TL106 isolated from Tibetan pigs on probiotic potential and intestinal microbes in weaned piglets. Microbiol Spectr. 2022;10:e01205-21. https://doi.org/10.1128/spectrum.01205-21.
4. Ngalimat MS, Yahaya RSR, Baharudin MMAA, Yaminudin SM, Karim M, Ahmad SA, Sabri S. A review on the biotechnological applications of the operational group Bacillus amyloliquefaciens. Microorganisms. 2021;9:614. https://doi.org/10.3390/microorganisms9030614.
5. Rani N, Singh P, Kumar S, Kumar P, Bhankar V, Kumar K. Plant-mediated synthesis of nanoparticles and their applications: a review. Mater Res Bull. 2023;163:112233. https://doi.org/10.1016/j.materresbull.2023.112233.
6. Hano C, Abbasi BH. Plant-based green synthesis of nanoparticles: production, characterization and applications. Biomolecules. 2022;12:31. https://doi.org/10.3390/biom12010031.
7. Singh NA, Narang J, Garg D, Jain V, Payasi D, Suleman S, Swami RK. Nanoparticles synthesis via microorganisms and their prospective applications in agriculture. Plant Nano Biol. 2023;5:100047. https://doi.org/10.1016/j.plana.2023.100047
8. Wang Z, Chen C, Liu H, Hrynshpan D, Savitskaya T, Chen J, Chen J. Enhanced denitrification performance of Alcaligenes sp. TB by Pd stimulating to produce membrane adaptation mechanism coupled with nanoscale zero-valent iron. Sci Total Environ. 2020;708:135063. https://doi.org/10.1016/j.scitotenv.2019.135063.
9. Rathore C, Yadav VK, Amari A, Meena A, Chinedu Egbosiuba T, Verma RK, Mahdhi N, Choudhary N, Sahoo DK, Chundawat RS, Patel A. Synthesis and characterization of titanium dioxide nanoparticles from Bacillus subtilis MTCC 8322 and its application for the removal of methylene blue and orange G dyes under UV light and visible light. Front Bioeng Biotechnol. 2024;11:1323249. https://doi.org/10.3389/fbioe.2023.1323249.
10. Fouda A, Hassan SED, Saied E, Azab MS. An eco-friendly approach to textile and tannery wastewater treatment using maghemite nanoparticles (γ-Fe₂O₃-NPs) fabricated by Penicillium expansum strain (Kw). J Environ Chem Eng. 2021;9:104693. https://doi.org/10.1016/j.jece.2020.104693.
11. Fouda A, Eid AM, Abdelkareem A, Said HA, El-Belely EF, Alkhalifah DHM, Alshallash KS, Hassan SED. Phyco-synthesized zinc oxide nanoparticles using marine macroalgae, Ulva fasciata Delile, characterization, antibacterial activity, photocatalysis, and tanning wastewater treatment. Catalysts. 2022;12:756. https://doi.org/10.3390/catal12070756.
12. Al Malki JS, Hussien NA, Akkad LM, Al Thurmani SO, Al Motiri AE. Green synthesis of silver and titanium oxide nanoparticles using tea and eggshell wastes, their characterization, and biocompatibility evaluation. Sustainability. 2023;15:11858. https://doi.org/10.3390/su151511858.
13. Al-Asbahi MG, Al-Ofiry BA, Saad FA, Alnehia A, Al-Gunaid MQ. Silver nanoparticles biosynthesis using mixture of Lactobacillus sp. and Bacillus sp. growth and their antibacterial activity. Sci Rep. 2024;14:10224. https://doi.org/10.1038/s41598-024-59936-1.
14. Mondal AH, Yadav D, Mitra S, Mukhopadhyay K. Biosynthesis of silver nanoparticles using culture supernatant of Shewanella sp. ARY1 and their antibacterial activity. Int J Nanomedicine. 2020;15:8295–8310. https://doi.org/10.2147/IJN.S274535.
15. Shanthi S, Jayaseelan BD, Velusamy P, Vijayakumar S, Chih CT, Vaseeharan B. Biosynthesis of silver nanoparticles using a probiotic Bacillus licheniformis Dahb1 and their antibiofilm activity and toxicity effects in Ceriodaphnia cornuta. Microb Pathog. 2016;93:70–77. https://doi.org/10.1016/j.micpath.2016.01.014.
16. Mohmed AA, Saad E, Fouda A, Elgamal MS, Salem SS. Extracellular biosynthesis of silver nanoparticles using Aspergillus sp. and evaluation of their antibacterial and cytotoxicity. J Appl Life Sci Int. 2017;11:1–12. https://doi.org/10.9734/JALSI/2017/33491.
17. Dua TK, Giri S, Nandi G, Sahu R, Shaw TK, Paul P. Green synthesis of silver nanoparticles using Eupatorium adenophorum leaf extract: characterizations, antioxidant, antibacterial and photocatalytic activities. Chem Pap. 2023;77:2947–2956. https://doi.org/10.1007/s11696-023-02676-9.
18. Wei W, Yu D, Huang Q. Preparation of Ag/TiO₂ nanocomposites with controlled crystallization and properties as a multifunctional material for SERS and photocatalytic applications. Spectrochim Acta Part A Mol Biomol Spectrosc. 2020;243:118793. https://doi.org/10.1016/j.saa.2020.118793.
19. Yadav VK, Amari A, Wanale SG, Osman H, Fulekar MH. Synthesis of floral-shaped nanosilica from coal fly ash and its application for the remediation of heavy metals from fly ash aqueous solutions. Sustainability. 2023;15:2612. https://doi.org/10.3390/su15032612.
20. Mohd Yusof H, Abdul Rahman NA, Mohamad R, Zaidan UH, Samsudin AA. Biosynthesis of zinc oxide nanoparticles by cell-biomass and supernatant of Lactobacillus plantarum TA4 and its antibacterial and biocompatibility properties. Sci Rep. 2020;10:19996. https://doi.org/10.1038/s41598-020-76402-w.
21. Keshari AK, Srivastava R, Singh P, Yadav VB, Nath G. Antioxidant and antibacterial activity of silver nanoparticles synthesized by Cestrum nocturnum. J Ayurveda Integr Med. 2020;11:37–44. https://doi.org/10.1016/j.jaim.2017.11.003.
22. Almutairi Z. Effect of silver nanoparticles on seed germination of crop plants. J Adv Agric. 2015;4:4295. https://doi.org/10.24297/jaa.v4i1.4295.
23. Xin X, Zhao F, Rho JY, Goodrich SL, Sumerlin BS, He Z. Use of polymeric nanoparticles to improve seed germination and plant growth under copper stress. Sci Total Environ. 2020;745:141055. https://doi.org/10.1016/j.scitotenv.2020.141055.
24. International Seed Testing Association. International rules for seed testing: seed health testing methods is the supplement to chapter 7 international rules for seed testing; edition 2013; catalogued separately. 2013.
25. Oshone K, Gebeyehu S, Tesfaye K. Assessment of common bean (Phaseolus vulgaris L.) seed quality produced under different cropping systems by smallholder farmers in Eastern Ethiopia. Afr J Food Agric Nutr Dev. 2014;14:8566.
26. Ellis RH, Roberts EH. The quantification of ageing and survival in orthodox seeds. Seed Sci Technol. 1981;9(2).
27. Jimoh AA, Akpeji BH, Azeez SO, Ayipo YO, Abdulsalam ZA, Adebayo ZF, Ajao AT, Zakariyah AT, Elemike EE. Biosynthesis of Ag and TiO₂ nanoparticles and the evaluation of their antibacterial activities. Inorg Chem Commun. 2022;141:109503. https://doi.org/10.1016/j.inoche.2022.109503.
28. Rahman MS, Chakraborty A, Kibria A, Hossain MJ. Effects of silver nanoparticles on seed germination and growth performance of pea (Pisum sativum). Plant Nano Biol. 2023;5:100042. https://doi.org/10.1016/j.plana.2023.100042.
29. Khan S, Zahoor M, Khan RS, Ikram M, Islam NU. The impact of silver nanoparticles on the growth of plants: the agriculture applications. Heliyon. 2023;9:e16928. https://doi.org/10.1016/j.heliyon.2023.e16928.
30. Fouad H, Hongjie L, Yanmei D, Baoting Y, El-Shakh A, Abbas G, Jianchu M. Synthesis and characterization of silver nanoparticles using Bacillus amyloliquefaciens and Bacillus subtilis to control filarial vector Culex pipiens pallens and its antimicrobial activity. Artif Cells Nanomed Biotechnol. 2017;45:1369–1378. https://doi.org/10.1080/21691401.2016.1241793.
31. Hieu HN, Trang DTH, Hien VTT, Nghia NV, Lam NT, Nguyen TMD. Microorganism-mediated green synthesis of silver nanoparticles using Aspergillus niger and Bacillus megaterium. Dig J Nanomater Biostruct. 2022;17:359–367. https://doi.org/10.15251/DJNB.2022.171.359.
32. Quevedo AC, Guggenheim E, Briffa SM, Adams J, Lofts S, Kwak M, Lee TG, Johnston C, Wagner S, Holbrook TR, Hachenberger YU. UV-Vis spectroscopic characterization of nanomaterials in aqueous media. JoVE. 2021;176:e61764. https://doi.org/10.3791/61764.
33. Titus D, Samuel EJJ, Roopan SM. Nanoparticle characterization techniques. In: Green synthesis, characterization and applications of nanoparticles. Elsevier; 2019. p. 303–319. https://doi.org/10.1016/B978-0-08-102579-6.00012-5.
34. Uthiravel V, Narayanamurthi K, Raja V, Anandhabasker S, Kuppusamy K. Green synthesis and characterization of TiO₂ and Ag-doped TiO₂ nanoparticles for photocatalytic and antimicrobial applications. Inorg Chem Commun. 2024;170:113327. https://doi.org/10.1016/j.inoche.2024.113327.
35. Rathore R, Choudhary KK, Kaurav N. The structural and optical properties of zinc-oxide nanomaterials synthesized via thermal decomposition method. J Phys Conf Ser. 2023;2603:012003. https://doi.org/10.1088/1742-6596/2603/1/012003.
36. Awad MA, Alanazi MM, Hendi AA, Virk P, Alrowaily AW, Bahlool T, Alhakami FS, Aouaini F, Ibrahim EM. Potential role of ‘green’ synthesized titanium dioxide nanoparticles in photocatalytic applications. Crystals. 2022;12:1639. https://doi.org/10.3390/cryst12111639.
37. Abd Elkodous M, El-Khawaga AM, Abouelela MM, Abdel Maksoud MIA. Cocatalyst loaded Al-SrTiO₃ cubes for Congo red dye photo-degradation under wide range of light. Sci Rep. 2023;13:6331. https://doi.org/10.1038/s41598-023-33249-1.
38. Herrero YR, Camas KL, Ullah A. Characterization of biobased materials. In: Advanced applications of biobased materials. Elsevier; 2023. p. 111–143. https://doi.org/10.1016/B978-0-323-91677-6.00005-2.
39. Carrapico A, Martins MR, Caldeira AT, Mirao J, Dias L. Biosynthesis of metal and metal oxide nanoparticles using microbial cultures: mechanisms, antimicrobial activity and applications to cultural heritage. Microorganisms. 2023;11:378. https://doi.org/10.3390/microorganisms11020378.
40. Petreanu I, Niculescu VC, Enache S, Iacob C, Teodorescu M. Structural characterization of silica and amino-silica nanoparticles by Fourier transform infrared (FTIR) and Raman spectroscopy. Anal Lett. 2023;56:390–403. https://doi.org/10.1080/00032719.2022.2083144.
41. Jain AS, Pawar PS, Sarkar A, Junnuthula V, Dyawanapelly S. Bionanofactories for green synthesis of silver nanoparticles: toward antimicrobial applications. Int J Mol Sci. 2021;22:11993. https://doi.org/10.3390/ijms222111993.
42. Usman Khan M, Rehman W, Bibi S, Alanazi MM, Alanazi AS, Rasheed L, Khan S, Tariq Gillani SU, Tauqeer A. Synthesis, characterization, and antimicrobial and nematicidal activities of chitosan-based silver-doped titanium dioxide. ACS Omega. 2023;8:19341–19350. https://doi.org/10.1021/acsomega.3c00068.
43. Gupta J, Arya S, Verma S, Singh A, Sharma A, Singh B, Sharma R. Performance of template-assisted electrodeposited Copper/Cobalt bilayered nanowires as an efficient glucose and uric acid sensor. Mater Chem Phys. 2019;238:121969. https://doi.org/10.1016/j.matchemphys.2019.121969.
44. Ahmed I, Mir FA, Banday JA. Synthesis of metal and metal oxide nanoparticles using plant extracts characterization and applications. BioNanoScience. 2023;13:1541–1557. https://doi.org/10.3390/jfb13040274.
45. Filippov SK, Khusnutdinov R, Murmiliuk A, Inam W, Zakharova LY, Zhang H, Khutoryanskiy VV. Dynamic light scattering and transmission electron microscopy in drug delivery: a roadmap for correct characterization of nanoparticles and interpretation of results. Mater Horiz. 2023;10:5354–5370. https://doi.org/10.1039/D3MH00717K.
46. Selim S, Badr BM, Moustafa MH, Saied E, Elkady FM, Salama A, Aloufi AS, Algamdi NA, Alzahofi NN, Abada E, Alshammari MS. Green biosynthesis of bimetallic silver titanium dioxide nanoparticles using Pluchea indica with their anticancer, antimicrobial, and antioxidant activities. Sci Rep. 2025;15:26735. https://doi.org/10.1038/s41598-025-10349-8.
47. Heydari Z, Ghadam P. Biosynthesis of titanium dioxide nanoparticles by the aqueous extract of Juglans regia green husk. Mater Proc. 2023;14:43. https://doi.org/10.3390/IOCN2023-14482.
48. Joshi SM, De Britto S, Jogaiah S, Ito SI. Mycogenic selenium nanoparticles as potential new generation broad spectrum antifungal molecules. Biomolecules. 2019;9:419. https://doi.org/10.3390/biom9090419.
49. Kaliva M, Vamvakaki M. Nanomaterials characterization. In: Polymer science and nanotechnology. Elsevier; 2020. p. 401–433. https://doi.org/10.1016/B978-0-12-816806-6.00017-0.
50. Rao TN, Babji P, Ahmad N, Khan RA, Hassan I, Shahzad SA, Husain FM. Green synthesis and structural classification of Acacia nilotica mediated-silver doped titanium oxide (Ag/TiO₂) spherical nanoparticles: assessment of its antimicrobial and anticancer activity. Saudi J Biol Sci. 2019;26:1385–1391. https://doi.org/10.1016/j.sjbs.2019.09.005.
51. Ibrahim S, Ali J, Rajasekar S, Chakravarthy NS, Varsha, Singh MS, Kumar V, Saruchi. Synthesis, characterization of Ag/TiO₂ nanocomposite: its anticancer and anti-bacterial activities. Glob Nest J. 2022;24:262–266. https://doi.org/10.30955/gnj.004255.
52. Pudlarz AM, Ranoszek-Soliwoda K, Czechowska E, Tomaszewska E, Celichowski G, Grobelny J, Szemraj J. A study of the activity of recombinant Mn-superoxide dismutase in the presence of gold and silver nanoparticles. Appl Biochem Biotechnol. 2019;187:1551–1568. https://doi.org/10.1007/s12010-018-2896-y.
53. Alam H, Khatoon N, Khan MA, Husain SA, Saravanan M, Sardar M. Synthesis of selenium nanoparticles using probiotic bacteria Lactobacillus acidophilus and their enhanced antimicrobial activity against resistant bacteria. J Cluster Sci. 2020;31:1003–1011. https://doi.org/10.1007/s10876-019-01705-6.
54. Nethravathi PC, Nagaraju G, Suresh D. TiO₂ and Ag-TiO₂ nanomaterials for enhanced photocatalytic and antioxidant activity: green synthesis using Cucumis melo juice. Mater Today Proc. 2022;49:841–848. https://doi.org/10.1016/j.matpr.2021.05.670.
55. Prazak R, Swiecilo A, Krzepilko A, Michalek S, Arczewska M. Impact of Ag nanoparticles on seed germination and seedling growth of green beans in normal and chill temperatures. Agriculture. 2020;10:312. https://doi.org/10.3390/agriculture10080312.
56. Haleke Besaye B, Galgaye GG. Impact of common bean (Phaseolus vulgaris L.) genotypes on seed yield, and seed quality at different locations of Eastern Ethiopia. Cogent Food Agric. 2022;8:2115674. https://doi.org/10.1080/23311932.2022.2115674.
57. Iqbal T, Irfan F, Afsheen S, Zafar M, Naeem S, Raza A. Synthesis and characterization of Ag–TiO₂ nano-composites to study their effect on seed germination. Appl Nanosci. 2021;11:2043–2057. https://doi.org/10.1007/s13204-021-01912-6.