Characterisation of Lectin from Pisum sativum and Agaricus bisporus and their Immunomodulatory Effects on Earthworm Coelomocytes (Immunomodulatory studies)


Authors : Yogitha N sagar; Dr. Preetha Nair

Volume/Issue : Volume 7 - 2022, Issue 3 - March

Google Scholar : https://bit.ly/3IIfn9N

Scribd : https://bit.ly/3uK2aIz

DOI : https://doi.org/10.5281/zenodo.6400167

The paper is focused on lectin characterisation from Pisum sativum and Agaricus bisporous by different experimental methods and assays such as antimicrobial effect, Haemagglutination and Sugar specificity. Lectins recognise cellular and molecular moieties and attach them by their sugar specificity. These are highly specific to carbohydrate moieties and the binding is reversible. Invertebrate system sample was taken to check the immunomodulatory effect of lectins. Trypan blue test for cell viability and catalase assay for antioxidant mechanism were assessed in this respect. And presence of lectin restores cell viability and nullifies free radical mediated toxicity. This paper will be valuable source for more studies of lectins and other related area of research.

Keywords : Immunomodulatory, Coelomocytes, Lectin, Stress Induced, PBS, HBSS, Trypan Blue Assay, Catalase Test.

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