Authors :
Zenas Chisom Agubata; Stanley Chukwudozie Onuoha; Dauphin Dighitoghi Moro; Miriam Oluchi Ukhureigbe; Sodunke Damilola Esther
Volume/Issue :
Volume 8 - 2023, Issue 11 - November
Google Scholar :
https://tinyurl.com/msk5bxu6
Scribd :
https://tinyurl.com/y2u34hsn
DOI :
https://doi.org/10.5281/zenodo.10426965
Abstract :
The bacteria community in the rhizosphere
region is critical for the growth of plants and the
formation of soil organic matter. This study focuses on
the identification of rhizospheric soil bacteria from rice
varieties grown under greenhouse environment. Samples
of Oryza sativa and Oryza glabberima were collected from
the National Cereals Research Institute (NCRI)
Umuahia and Rice mill at Kpirikpiri Ebonyi
State. Oryza sativa grew more rapidly than Oryza
glabberima because of the variations in rice species. The
rice seeds were planted in nursery and transplanted in
buckets under greenhouse conditions (75% humidity
and at room temperature 25°C). The adhering soil
samples from the rhizosphere region were gotten from
the roots and 1g of soil weighed aseptically and dissolve
in sterile distilled water. An aliquot was picked for serial
dilution. The serially diluted aliquot was inoculated on
nutrient agar and incubated. Individual colonies were
subcultured. Microbiological, biochemical and sugar
fermentation tests were able to classify the isolates from
Oryza sativa into Azotobacter spp., and Bacillus spp., and
Oryza glabberima into Bacillus spp and Pseudomonas
spp. respectively. This study showed that there are a lot
of soil bacteria in the rhizospheric region of Oryza sativa
than Oryza glabberima because it is an improved variety
and enhance the utilization of available nutrients and
minerals by microbial interaction which enable rapid
growth of the plant, therefore, Oryza sativa should be
cultivated more for high yield and large-scale
agricultural production.
Keywords :
Oryza Sativa, Oryza Glabberima, Rhizosphere, Bacteria, Greenhouse, Nerica5, M306.
The bacteria community in the rhizosphere
region is critical for the growth of plants and the
formation of soil organic matter. This study focuses on
the identification of rhizospheric soil bacteria from rice
varieties grown under greenhouse environment. Samples
of Oryza sativa and Oryza glabberima were collected from
the National Cereals Research Institute (NCRI)
Umuahia and Rice mill at Kpirikpiri Ebonyi
State. Oryza sativa grew more rapidly than Oryza
glabberima because of the variations in rice species. The
rice seeds were planted in nursery and transplanted in
buckets under greenhouse conditions (75% humidity
and at room temperature 25°C). The adhering soil
samples from the rhizosphere region were gotten from
the roots and 1g of soil weighed aseptically and dissolve
in sterile distilled water. An aliquot was picked for serial
dilution. The serially diluted aliquot was inoculated on
nutrient agar and incubated. Individual colonies were
subcultured. Microbiological, biochemical and sugar
fermentation tests were able to classify the isolates from
Oryza sativa into Azotobacter spp., and Bacillus spp., and
Oryza glabberima into Bacillus spp and Pseudomonas
spp. respectively. This study showed that there are a lot
of soil bacteria in the rhizospheric region of Oryza sativa
than Oryza glabberima because it is an improved variety
and enhance the utilization of available nutrients and
minerals by microbial interaction which enable rapid
growth of the plant, therefore, Oryza sativa should be
cultivated more for high yield and large-scale
agricultural production.
Keywords :
Oryza Sativa, Oryza Glabberima, Rhizosphere, Bacteria, Greenhouse, Nerica5, M306.
