Authors :
Md. Ariful Islam; Mohammad Ataur Rahman; Tanbin Akter; Md. Monirul Islam; Muhammad Abdus Salam; Sharmin Sultana Misty; Mohammad Anwar Hossain; Md. Ashraful Haque
Volume/Issue :
Volume 8 - 2023, Issue 3 - March
Google Scholar :
https://bit.ly/43uxUln
Scribd :
https://bit.ly/3Agy5TU
DOI :
https://doi.org/10.5281/zenodo.7852613
Abstract :
Xanthomonas oryzae pv. oryzae (Xoo) is the
disease-causing agent behind bacterial leaf blight (BLB)
affecting the leaves at the plant growth phases of rice. It
produces noticeable symptoms in infected rice, such as
leaf blight, which appears on the leaves of rice plants
adjacent to the leaf tips and margins. This study intends
to explore bacterial Xoo isolates from 25 regions in
Bangladesh through biochemical and molecular
characterization. The bacterial isolates were grown in
petri dishes on Peptone Sucrose Agar (PSA) medium,
and the isolates had colonies ranging in size from 0.08
mm to 1.1 mm, and their color ranged from light yellow
to yellow with a circular margin. Majority of the
isolates produced raised and slimy colonies. The
bacteria were then subjected to different biochemical
tests resulting in positive for KOH (3%) test, gelatin
liquefaction, catalase test, and while, negative for starch
hydrolysis, gram reaction, and oxidase tests. Moreover,
the DNA of Xoo-indicating bacteria was isolated, and
among the 29 isolates, 15 Xoo-bacteria were confirmed
through PCR amplification using a specific primer,
Xoo2976. All 15 virulent Xoo-bacteria were subjected to
a pathogenicity test on 3 rice varieties, BRRI DHAN49,
IRBB66, and IRRI 154 yielded significant results in
terms of disease severity. Xoo17 exhibits the highest
virulence from the pathogenicity test. The study's
outcomes may be utilized for screening BLB-resistant
rice in varietal development research.
Keywords :
Bacterial Leaf Blight; Characterization; Resistance; Xoo2976; Xanthomonas oryzae pv. Oryzae.
Xanthomonas oryzae pv. oryzae (Xoo) is the
disease-causing agent behind bacterial leaf blight (BLB)
affecting the leaves at the plant growth phases of rice. It
produces noticeable symptoms in infected rice, such as
leaf blight, which appears on the leaves of rice plants
adjacent to the leaf tips and margins. This study intends
to explore bacterial Xoo isolates from 25 regions in
Bangladesh through biochemical and molecular
characterization. The bacterial isolates were grown in
petri dishes on Peptone Sucrose Agar (PSA) medium,
and the isolates had colonies ranging in size from 0.08
mm to 1.1 mm, and their color ranged from light yellow
to yellow with a circular margin. Majority of the
isolates produced raised and slimy colonies. The
bacteria were then subjected to different biochemical
tests resulting in positive for KOH (3%) test, gelatin
liquefaction, catalase test, and while, negative for starch
hydrolysis, gram reaction, and oxidase tests. Moreover,
the DNA of Xoo-indicating bacteria was isolated, and
among the 29 isolates, 15 Xoo-bacteria were confirmed
through PCR amplification using a specific primer,
Xoo2976. All 15 virulent Xoo-bacteria were subjected to
a pathogenicity test on 3 rice varieties, BRRI DHAN49,
IRBB66, and IRRI 154 yielded significant results in
terms of disease severity. Xoo17 exhibits the highest
virulence from the pathogenicity test. The study's
outcomes may be utilized for screening BLB-resistant
rice in varietal development research.
Keywords :
Bacterial Leaf Blight; Characterization; Resistance; Xoo2976; Xanthomonas oryzae pv. Oryzae.
