Tinospora cordifolia is a priority medicinal plant with significant therapeutic value, particularly in Indian medicine. However, for quick multiplication, this plant requires a regeneration technique. T. cordifolia nodal explants were used to design a successful regeneration technique.When nodal segments were cultivated on MS media enriched with BAP and 2-iP, a high frequency of multiple shoot development was produced. On this medium and hormonal concentration, the maximum mean number of shoots per nodal explant, the longest shoot length, and an 86 percent response were attained. Tinospora cordifolia (Willd) Miers has a successful in vitro micropropagation strategy based on shoot tip explants. Young and mature shoot tip (YST and MST) explants from two different-aged plant sources (15 days and 3 years) were treated simultaneously in the micropropagation methodology to develop and evaluate their effectiveness.6-benzyladenine (BA) and kinetin (KN) performed better in bud break and shoot growth than the other cytokinins and synergetic treatments, at both individual and combined dosages.Multiple shoot induction and development were improved when the auxin indole-3-acetic acid (IAA) was added to the appropriate cytokinin doses of BA and KN.Additional gibberellic acid and an antioxidant (ascorbic acid) supplementation improved shoot bud induction, shot number, and shoot length considerably.Also, this review article gives an idea of application of Tinosporacordifolia and how to enhanced micropropagation by the combination of various Plant Growth Regulators and its concentration.