Authors :
Dr. Aastha Gajavalli; Dr. Suchetha A.; Dr. Sapna N.; Dr. Darshan B. M.; Dr. Apoorva S. M.
Volume/Issue :
Volume 10 - 2025, Issue 11 - November
Google Scholar :
https://tinyurl.com/yur6tu4w
Scribd :
https://tinyurl.com/5hc95v4y
DOI :
https://doi.org/10.38124/ijisrt/25nov1404
Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.
Abstract :
Aim-
Aerosols that are generated in dental environment during ultrasonic scaling may cause problems for health of dentists
and patients. It is therefore important for dentists to adopt best practices in reducing the risk of infectious disease through
aerosols by evaluating the aerosol mitigation interventions. Therefore, the aim of the study was to compare the efficacy of
two fumigating agents in reducing aerosol microbial count (CFU) before and after ultrasonic scaling at different time
intervals.
Methods-
Ultrasonic scaling was performed on 30 systemically and periodontally healthy subjects. 10 patients each were divided
into 3 groups- Group 1- (control) No fumigation, Group 2- fumigation using agent 1 (ammonium compound +silver nitrate),
Group 3- fumigation using agent 2(hydrogen peroxide +silver nitrate). The active air sampling method was used to collect
aerosols using MAS100 sampler at time intervals- before, immediately after, one hour and two hours later ultrasonic scaling
(T0, T1, T2, T3).
Results-
Fumigating agent 2 demonstrated superior performance in maintaining lower CFU compared to other groups
suggesting it to be a more effective agent for reducing microbial contamination in dental cabin.
Conclusion-
The study highlights the importance of using fumigating agents in dental cabins to control colony factor units (CFU)
levels before and after ultrasonic scaling.
Keywords :
Aerosols, Microorganisms, Fumigation, Ultrasonic Scaling.
References :
- Hinds, William C. (1999). Aerosol Technology (2nd ed.). Wiley - Interscience.
- Seinfeld J, Pandis S (1998). Atmospheric Chemistry and Physics: From Air Pollution to Climate Change (2nd ed.). Hoboken, New Jersey: John Wiley & Sons. p. 97
- Micik RE, Miller RL, Mazzarella MA, Ryge G. Studies on dental aerobiology, I: bacterial aerosols generated during dental procedures. J Dent Res 1969;48(1):49-56.
- Miller RL, Micik RE, Abel C, Ryge G. Studies of dental aerobiology, II: microbial splatter discharged from the oral cavity of dental patients. J Dent Res 1971; 50:621-5.
- Abel LC, Miller RL, Micik RE, Ryge G. Studies on dental aerobiology, IV: bacterial contamination of water delivered by dental units. J Dent Res 1971; 50:1567-9.
- Miller RL, Micik RE. Air pollution and its control in the dental office. Dent Clin North Am 1978; 22:453-76.
- Hinds WC. Aerosol technology: Properties, behavior, and measurement of airborne particles. New York: Wiley; 1982:6-8.
- Cottone JA, Terezhalmy GT, Molinari JA. Practical infection control in dentistry. Baltimore: Williams & Wilkins; 1996:139-40
- King TB, Muzzin KB, Berry CW, Anders LM. The effectiveness of an aerosol reduction device for ultrasonic scalers. J Periodontol 1997;68(1):45-9.
- Logothetis DD, Gross KB, Eberhart A, Drisko C. Bacterial airborne contamination with an air-polishing device. Gen Den 1988;36: 496-9.
- Bentley CD, Burkhart NW, Crawford JJ. Evaluating spatter and aerosol contamination during dental procedures. JADA 1994;125: 579-84.
- Legnani P, Checchi L, Pelliccioni GA, D’Achille C. Atmospheric contamination during dental procedures. Quintessence Int 994; 25:435-9. 25. Gross KB, Overman PR, Cobb C, Brockmann S. Aerosol generation by two ultrasonic scalers and one sonic scaler: a comparative study. J Dent Hyg 1992; 66:314-8
- King TB, Muzzin KB, Berry CW, Anders LM. The effectiveness of an aerosol reduction device for ultrasonic scalers. J Periodontol 1997;68(1):45-9.
- Muzzin KB, King TB, Berry CW. Assessing the clinical effectiveness of an aerosol reduction device for the air polisher. JADA 1999; 130:1354-9.
- Harrel SK. Clinical use of an aerosol-reduction device with ultrasonic scaler. Compend Cont Educ Dent 1996; 17:1185-93.
- Barnes JB, Harrel SK, Rivera-Hidalgo F. Blood contamination of the aerosols produced by the in vivo use of ultrasonic scalers. J Periodontol 1998; 69:434-8.
- Infection control recommendations for the dental office and the dental laboratory. ADA Council on Scientific Affairs and ADA Council on Dental Practice. JADA 1996,127:672-80.
- Pippen DJ, Verderame RA, Weber KK. Efficacy of face masks in preventing inhalation of airborne contaminants. J Oral Maxillofac Surg 1987; 45:319-23.
- Logothetis DD, Martinez-Welles JM. Reducing bacterial aerosol contamination with a chlorhexidine gluconate pre-rinse. JADA 1995;126: 1634-9.
- Fine DH, Korik I, Furgang D, et al. Assessing pre-procedural subgingival irrigation and rinsing with an antiseptic mouthrinse to reduce bacteremia. JADA 1996;127(5):641-2, 645-6.
- Harrel SK, Barnes JB, Rivera-Hidalgo F. Reduction of aerosols produced by ultrasonic scalers. J Periodontol 1996;67(1):28-32.
- Jacks ME. A laboratory comparison of evacuation devices on aerosol reduction. J Dent Hyg 2002;76(3):202-6.
- Klyn SL, Cummings DE, Richardson BW, Davis RD. Reduction of bacteria-containing spray produced during ultrasonic scaling. Gen Dent 2001;49(6):648-52.
- Harrel SK, Barnes JB, Rivera-Hidalgo F. Aerosol reduction during air polishing. Quintessence Int 1999; 30:623-8.
- Occupational exposure to bloodborne pathogens: OSHA—Final rule. Fed Regist 1991;56(235):64004-182.
- Kohn WG, Collins AS, Cleveland JL, Harte JA, Eklund KJ, Malvitz DM; Centers for Disease Control and Prevention (CDC). Guidelines for infection control in dental health-care settings: 2003. MMWR Recomm Rep 2003;52(RR-17):16-7.
- Kumbargere Nagraj S, Eachempati P, Paisi M, Nasser M, Sivaramakrishnan G, Verbeek JH. Interventions to reduce contaminated aerosols produced during dental procedures for preventing infectious diseases. Cochrane Database Syst Rev. 2020 Jul
- Dominiak, M.; Różyło-Kalinowska, I.; Gedrange, T.; Konopka, T.; Hadzik, J.; Bednarz, W.; Matys, J.; Lella, A.; Rayad, S.; Maksymowicz, R.; et al. COVID-19 and Professional Dental Practice. The Polish Dental Association Working Group Recommendations for Procedures in Dental Office during an Increased Epidemiological Risk. J. Stomatol. 2020, 73, 1–10.
- WHO. Infection Prevention and Control of Epidemic-and Pandemic Prone Acute Respiratory Infections in Health Care; WHO: Geneva, Switzerland, 2014
- Gumru, B.; Tarcin, B.; Idman, E. Cross-Contamination and Infection Control in Intraoral Digital Imaging: A Comprehensive Review. Oral Radiol. 2021, 37, 180–188.
- Bromberg, N.; Brizuela, M. Preventing Cross-Infection in the Dental Office; StatPearls Publishing: Tampa, FL, USA, 2023.
- Darge, A.; Kahsay, A.G.; Hailekiros, H.; Niguse, S.; Abdulkader, M. Bacterial Contamination and Antimicrobial Susceptibility Patterns of Intensive Care Units Medical Equipment and Inanimate Surfaces at Ayder Comprehensive Specialized Hospital, Mekelle, Northern Ethiopia. BMC Res. Notes 2019, 12, 621.
- Saadi, S.; Allem, R.; Sebaihia, M.; Merouane, A.; Bakkali, M. Bacterial Contamination of Neglected Hospital Surfaces and Equipment in an Algerian Hospital: An Important Source of Potential Infection. Int. J. Environ. Health Res. 2022, 32, 1373–1381.
- Oyeyemi A, Adesina A, Ogoina D. Fumigation of Schools for COVID-19 Prevention in Nigeria: The Need for a Rethink. Am J Trop Med Hyg. 2020; 103 (4): 1370–1371
- Srikanth P., Sudharsanam S., Steinberg R. Bio-aerosols in indoor environment: composition, health effects and analysis. Ind. J. Med. Biol. 2008; 26:302–312.
- Yamamoto, N., D. Schmechel, B. T. Chen, W. G. Lindsley, and J. Peccia. 2011. Comparison of quantitative airborne fungi measurements by active and passive sampling methods. J. Aerosol Sci. 42 (8):499–507.
- Therkorn, J., N. Thomas, J. Scheinbeim, and G. Mainelis. 2017b. Field performance of a novel passive bioaerosol sampler using polarized ferroelectric polymer films. Aerosol Sci. Technol. 51 (7):787–800
- Zhen, S. Q., K. J. Li, L. H. Yin, M. S. Yao, H. L. Zhang, L. S. Chen, M. H. Zhou, and X. D. Chen. 2009. A comparison of the efficiencies of a portable biostage impactor and a Reuter centrifugal sampler (RCS) high flow for measuring airborne bacteria and fungi concentrations. J. Aerosol Sci. 40 (6):503–513.
- Yao, M., and G. Mainelis. 2006. Investigation of cut-off sizes and collection efficiencies of portable microbial samplers. Aerosol Sci. Technol. 40 (8):595–606.
- Chen, H., and M. Yao. 2018. A high-flow portable biological aerosol trap (highbiotrap) for rapid microbial detection. J. Aerosol Sci. 117:212–223.
- Chang, C. W., and F. C. Chou. 2011. Assessment of bioaerosol sampling techniques for viable Legionella pneumophila by ethidium monoazide quantitative PCR. Aerosol Sci. Technol. 45 (3):343–351.
- Chang, C. W., and P. Y. Hung. 2012. Evaluation of sampling techniques for detection and quantification of airborne legionellae at biological aeration basins and shower rooms. J. Aerosol Sci. 48:63–74.
- Grinshpun, S. A., M. P. Buttner, G. Mainelis, and K. Willeke. 2015. Sampling for airborne microorganisms. In Manual of environmental microbiology, ed. M.V. Yates, C. H. Nakatsu, R.V. Miller, and S. D. Pillai, 3.2.2-1-3.2.2-17. Washington, DC: American Society for Microbiology.
- Macher, J. M., and M. W. First. 1983. Reuter centrifugal air sampler: Measurement of effective airflow rate and collection efficiency. Appl. Environ. Microbial. 45:1960–1962
- Zohra R, TALAT N, AZIZ S- A Comparative Study of Operation Theatre Disinfection by Fumigation Using Different Compounds. Pakistan Journal of Neurological Surgery. 2017;21(1):32-6.
- Boccia, G.; Di Spirito, F,D’Ambrosio, F.; De Caro, F.; Pecora, D,Giorgio, R.; Fortino, L.; Longanella, W.;Franci, G.; Santella, B.; et al. Microbial Air Contamination in a Dental Setting Environment and Ultrasonic Scaling in Periodontally Healthy Subjects: An Observational Study. Int. J. Environ.Res. Public Health 2023, 20, 2710.
- Matys, J., Gedrange, T., Dominiak, M., & Grzech-Leśniak, K. (2023). The Impact of Hydrogen Peroxide (H2O2) Fumigation on Bacterial Levels in Dental Office Environments: A Randomized Clinical Trial Investigation. Journal of Clinical Medicine, 12(24),2023
- Akbari M, Atoof F, Nazari-Alam A, Fatemi Nasab ZS, Miranzadeh MB, Mirzaei N. Assessment of bacterial bioaerosols and particulate matters characteristics in the indoor air of dentistry clinics. Int Arch Health Sci. 2023 Jan 1;10(3):130-6.
- Del Álamo C, Vázquez-Calvo Á, Sanchiz Á, Rodríguez-Caravaca G, Martín R, Hernáez B, Méndez-Vigo-Carranza P, Sánchez García-Casarrubios J, Alcamí A, Pérez-Díaz JL. Fast Air-to-Liquid Sampler Detects Surges in SARS-CoV-2 Aerosol Levels in Hospital Rooms. Int J Environ Res Public Health. 2022 Dec 29;20(1):576.
- Napoli C, Marcotrigiano V, Montagna MT. Air sampling procedures to evaluate microbial contamination: a comparison between active and passive methods in operating theatres. BMC Public Health. 2012 Aug 2;12:594.
- Gandolfi, M.G.; Zamparini, F.; Spinelli, A.; Sambri, V.; Prati, C. Risks of Aerosol Contamination in Dental Procedures during the Second Wave of COVID-19—Experience and Proposals of Innovative IPC in Dental Practice. Int. J. Environ. Res. Public Health 2020, 17, 8954
- Rutala WA, Weber DJ. Surface disinfection: Should we do it? J Hosp Infect 2001;48: S64-8
- Rutala WA. APIC guidelines for selection and use of disinfectants. Am J Infect Control 1996; 24:313-42
- Pierson J. Choosing a disinfectant for hospital environment. Indoor Environment Connections. 2009;10.
- Singh M, Sharma R, Gupta PK, Rana JK, Sharma M, Taneja N. Comparative efficacy evaluation of disinfectants routinely used in hospital practice: India. Indian J Crit Care Med 2012; 516:123-9.
- Stawarz-Janeczek M, Kryczyk-Poprawa A, Muszyńska B, Opoka W, Pytko-Polończyk J. Disinfectants Used in Stomatology and SARS-CoV-2 Infection. Eur J Dent. 2021 May;15(2):388-400.
Aim-
Aerosols that are generated in dental environment during ultrasonic scaling may cause problems for health of dentists
and patients. It is therefore important for dentists to adopt best practices in reducing the risk of infectious disease through
aerosols by evaluating the aerosol mitigation interventions. Therefore, the aim of the study was to compare the efficacy of
two fumigating agents in reducing aerosol microbial count (CFU) before and after ultrasonic scaling at different time
intervals.
Methods-
Ultrasonic scaling was performed on 30 systemically and periodontally healthy subjects. 10 patients each were divided
into 3 groups- Group 1- (control) No fumigation, Group 2- fumigation using agent 1 (ammonium compound +silver nitrate),
Group 3- fumigation using agent 2(hydrogen peroxide +silver nitrate). The active air sampling method was used to collect
aerosols using MAS100 sampler at time intervals- before, immediately after, one hour and two hours later ultrasonic scaling
(T0, T1, T2, T3).
Results-
Fumigating agent 2 demonstrated superior performance in maintaining lower CFU compared to other groups
suggesting it to be a more effective agent for reducing microbial contamination in dental cabin.
Conclusion-
The study highlights the importance of using fumigating agents in dental cabins to control colony factor units (CFU)
levels before and after ultrasonic scaling.
Keywords :
Aerosols, Microorganisms, Fumigation, Ultrasonic Scaling.