Introduction: The Enterococcus faecalis and Candida albicans are resistant species in infected root canals causing treatment failures. Sodium hypochlorite is an irrigant with many disadvantages. Thus, a harmless irrigant can always be a choice. Objectives: The aim of the study is to evaluate and compare the antimicrobial activity of Piper betel leaf, Phyllanthus emblica, Lawsonia inermis as an irrigating solution. Materials & Method: The extract of Piper betel leaf, Phyllanthus emblica, Lawsonia inermis were prepared and zones of inhibition were measured against E. faecalis and C. albicans and were compared with sodium hypochlorite and normal saline (Control group).Results: The results revealed that Phyllanthus emblica showed the highest antibacterial and 0.5% show anti fungal activity and highest zone of inhibition against E. Faecalis and C. Albicans among all the groups. Conclusion: Piper betel, Phyllanthus emblica, Lawsonia inermis shows effective antimicrobial and antifungal activity against E. Faecalis and C. Albicans.

Keywords : Microorganism, Irrigant, Zone of Inhibition