Authors : Nwafor, P. M.; Ezeanyanwu V. C.; Obodoeze, A. I.

Volume/Issue : Volume 9 - 2024, Issue 10 - October

Google Scholar : https://tinyurl.com/5dtkjuxy

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DOI : https://doi.org/10.38124/ijisrt/IJISRT24OCT1498

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Abstract : The purpose of this study is conducting a comparative study on the nutritional compositions of bitterleaf (Vernonia amygdalina) and oha leaf (Pterocarpus mildbraedii), focusing on its phytochemicals and micronutrients so as to determine their nutritional value, the bioactive compounds present in them and relate them to their health benefits. The collected leaf samples of V. amygdalina and P. mildbreadii were divided into three parts each, for the phytochemicals, vitamin and mineral determination. The oils of the grounded leaf samples were extracted using soxhlet extraction method and the phytochemical contents of the leaves were analyzed using GC-FID. Vitamin A and Vitamin E absorbance were measured at mg/L using a UV- spectrophotometer at different wavelength while Vitamin C was titrated and measured at mg/100ml. The samples were also digested for the mineral analysis and their filtrates were placed in an atomic absorption spectrophotometer and the absorbance of the different elements contained in the samples was measured at mg/L. The results indicated that bitter leaf was high in flavonoid, alkaloid and kaempferol having values of 15.877 ± 0.011, 15.577 ± 0.006 and 13.890 ± 0.004, respectively while the oha leaf was high in kaempferol, sapogenin and phenol having values of 16.873 ± 0.001, 15.125 ± 0.001 and 14.779 ± 0.001, respectively. The Vitamin A (mg/L),Vitamin E (mg/L) and Vitamin C (mg/100) values of bitter leaf and oha leaf were 18.579, 22.120,1174.493 and 20.323, 25.918, 1275.235 respectively. The mineral levels of V. amygdalina and P. mildbraedii revealed significant amounts of magnesium and potassium having higher values of 4.779mg/L, 3.265mg/L and 3.782mg/L, 2.712mg/L respectively. Iron and zinc were not present in the P. mildbreadii leaves while copper, iron and sodium were not present in the V. amygdalina leaves. The evaluation of the leaves of V. amygdalina and P. mildbreadii showed that the leaves had high phytochemical, mineral and vitamin contents. However, the difference between the two samples on their phytochemical and micronutrient composition had significant variations at p<0.05. The result of this analysis will aid to inform the consumers on the appropraite usage of this leaves for optimal nutritional yield. It should also be recommended by food nutritionist as part of a dietary plan so as to help boost the immune system and support other body processes.

Keywords : V. Amygdalina, P. Mildbreadii, Phytochemicals, Micronutrients, Soxhlet Extraction.

References :

1. Ali A. (2012). Screening of phytochemical compound and toxic proteinaceous protease indicator in some lesser known food based plants and their effects and potential application in food. International Journal of Food Science and Nutrition Engineering. 2(3), pp. 16-20.
2. Amabeoku G. J. (2009). Anti-diarrhoeal activity of geranium incanum burm. Leaf extract in mice. Ethnopharmacology. (123), 190-193.
3. Anderson G.D., (2004). Dynamic Chiropractic. Trends Food Science Technology. 4: 168175
4. AOAC. (2010) Official methods of analysis 19^th edition., Association of Official Analytical Chemists, Washington D. C U.S.A.
5. Chen Yu-Fen C. H., Chang M. S., Gou Y. P. and Huang, Y. C. (2010). Foam properties and detergent abilities of the saponins from Camellia oleifera. International Journal of Molecular Sciences. (11), pp. 4417-4425.
6. Danladi S., Hassan M. A., Masa’ud I. A. and Ibrahim U. I. (2018). Vernonia amygdalina; a mini review. Res. J. Pharm Technol. 11(9), pp. 4187-4190.
7. Dewanto V., Wu X and Liu R.H., (2002). Processed sweet corn has higher antioxidant activity. J. Agric. Food. Chem. (50), pp. 4959-4964.
8. Gūçlū -Ūstundag O. and Mazza G. (2007). Saponins, properties, application and processing. Critical Reviews in Food Science and Nutrition. (47), pp. 231-258.
9. Ijeh I.  I and Ejike C. E. C. C. (2011). Current perspectives on the medicinal potential of Vernonia amygdalina (Del). J. Med. Plant Res. 5(7), pp. 1051-1061.
10. Liu R.H. and Wang C. Y. (2008). Potential synergy of phytochemicals in cancer prevention: mechanism of action. J. Nutrition. (134), pp. 34795-34855.
11. Njan A. A., Adzu B., Agaba A. G., Byarugaba D., Díaz-Llera S. and Bangsberg D. R. (2008). The analgesic and antiplasmodial activities and toxicology of Vernonia amygdalina. Journal of Medicinal Food. 11(3), pp. 574-581
12. Ogunlesi A., Okolie H., Ndukwe O., Obidiebube E., Obasi C. and Enwerem J. (2017). Evaluation of nutritional and phytochemical compositions of two variants of bitter leaf (Vernonia amygdalina) accessions in Nigeria. Int. J. Res. Innovation Appl. Sci. 6 (12), 2454-6194.
13. Okonwu K., Akonye L. A. and Mensah S. L. (2018). Comparative studies on bioactive components of fluted pumpkin grown in three selected solid media. Journal of Experimental Agriculture International. 20 (2), pp. 1-10.
14. Okwu D. E. (2004). Phytochemicals and vitamin content of indigenous spices of South-eastern Nigeria. J. Sustain Agric. Environ. 6(1), pp. 30-37.
15. Quasie O., Zhang Y., Zhang H., Luo J. and Kong L. (2016). Four new steroid saponins with highly oxidized side chains from the leaves of Vernonia amygdalina. Journal of Phytochemistry Letters (15), 16–20.
16. Rao N. (2003). Bioactive phytochemicals in Indian foods and their potential health promotion and disease prevention. Asia Pacific Journal of Clinical Nutrition. 12(1), 9-22.
17. Sofowora A. (1993). Medical plants and traditional medicine in Africa. 2^nd ed., Spectrum books Ltd. Ibadan, Nigeria. Pp.71-73
18. Udensi E. U. and Anyika I. O. (2012). Phytochemical profile of some green leafy vegetables in South East, Nigeria. Nigerian Food Journal, (25), 1.