Comparative Study on the Impact of Lactic Acid Formation Over Time on Calcium Levels in Curd Made from Cow’s and Buffalo’s Milk


Authors : S. Yazhini; Neeharika L; Dhayanithi E; Santhanalakshmi V; Dr. Sivasakthi Balan

Volume/Issue : Volume 10 - 2025, Issue 7 - July


Google Scholar : https://tinyurl.com/3wmzewps

Scribd : https://tinyurl.com/3ezdd64u

DOI : https://doi.org/10.38124/ijisrt/25jul1614

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Abstract : This study explores the process of lactic acid production in milk through natural fermentation, primarily driven by Lactobacillus species, which convert lactose into lactic acid. This acidification lowers the milk’s pH, leading to the coagulation of proteins like casein and resulting in the distinct texture and flavor of fermented dairy products such as curd and yogurt. The research emphasizes that optimal temperature conditions (30°C to 40°C) are crucial for proper curd formation. It also highlights the differences between cow and buffalo milk, noting that cow milk acidifies more slowly even at optimal temperatures, making it more effective in retaining calcium. In contrast, buffalo milk ferments more rapidly, requiring careful temperature control to prevent excessive calcium loss. Additionally, the study stresses the importance of public awareness regarding nutrient preservation. Educational initiatives through schools, health programs, and media can encourage better food preparation and storage practices. Promoting the use of fresh, local ingredients and understanding food labels can help individuals, especially vulnerable groups, make informed dietary choices. Overall, the findings underscore the need to preserve nutrients during food processing and raise awareness so that essential nutrients can be retained or supplemented as needed for individual health and well-being.A comparison of lactic acid production at different temperatures shows that the inoculum becomes more active and multiplies more rapidly at temperatures optimal for Lactobacillus, leading to faster acid development. Conversely, at lower temperatures, the growth of the bacteria slows down, resulting in a slower rate of acid formation. Interestingly, buffalo milk tends to exhibit a quicker rise in acidity than cow milk, suggesting that fermentation occurs at a faster pace. However, this rapid acid production in buffalo milk can lead to increased calcium loss, as the rising acidity dissolves calcium phosphate, which is then released into the whey and removed during curd formation

Keywords : Milk, Lactic Acid, Curd, Temperature, Calcium.

References :

  1. Goyal, G. K., & Gandhi, D. N. (2009). Comparative analysis of cow and buffalo milk and their fermented products. Indian Dairyman, and Fox et al., Fundamentals of Cheese Science (2017
  2. Tamime & Robinson, Yoghurt: Science and Technology, 2007).
  3. (U.S. Department of Agriculture, 2020; National Institutes of Health, Office of Dietary Supplements)
  4. Ahmad et al., Buffalo Milk: Chemistry and Technology, 2013)
  5. (FDA, 2020)
  6. Fox et al., Fundamentals of Cheese Science, 2017)
  7. Marco et al., Annual Review of Food Science and Technology, 2017).
  8. (Ahmad et al., Buffalo Milk: Chemistry and Technology, 2013).
  9. Tamime & Robinson, Yoghurt: Science and Technology, 2007
  10. Tamime & Robinson, Yoghurt: Science and Technology, 2007; USDA FoodData Central).
  11. Ahmad et al., Buffalo Milk: Chemistry and Technology, 2013).
  12. Tamime & Robinson, Yoghurt: Science and Technology, 2007
  13. Tamime & Robinson, Yoghurt: Science and Technology, 2007; USDA FoodData Central).
  14. Goyal, G. K., & Gandhi, D. N. (2009). Comparative analysis of cow and buffalo milk and their fermented products. Indian Dairyman, and Fox et al., Fundamentals of Cheese Science (2017
  15. Fox et al., Fundamentals of Cheese Science, 2017
  16. (FAO/WHO, Guidelines on Nutrition and Food Processing, 2019)
  17. Farkas, Preservation and Shelf-Life Extension, 2004)

This study explores the process of lactic acid production in milk through natural fermentation, primarily driven by Lactobacillus species, which convert lactose into lactic acid. This acidification lowers the milk’s pH, leading to the coagulation of proteins like casein and resulting in the distinct texture and flavor of fermented dairy products such as curd and yogurt. The research emphasizes that optimal temperature conditions (30°C to 40°C) are crucial for proper curd formation. It also highlights the differences between cow and buffalo milk, noting that cow milk acidifies more slowly even at optimal temperatures, making it more effective in retaining calcium. In contrast, buffalo milk ferments more rapidly, requiring careful temperature control to prevent excessive calcium loss. Additionally, the study stresses the importance of public awareness regarding nutrient preservation. Educational initiatives through schools, health programs, and media can encourage better food preparation and storage practices. Promoting the use of fresh, local ingredients and understanding food labels can help individuals, especially vulnerable groups, make informed dietary choices. Overall, the findings underscore the need to preserve nutrients during food processing and raise awareness so that essential nutrients can be retained or supplemented as needed for individual health and well-being.A comparison of lactic acid production at different temperatures shows that the inoculum becomes more active and multiplies more rapidly at temperatures optimal for Lactobacillus, leading to faster acid development. Conversely, at lower temperatures, the growth of the bacteria slows down, resulting in a slower rate of acid formation. Interestingly, buffalo milk tends to exhibit a quicker rise in acidity than cow milk, suggesting that fermentation occurs at a faster pace. However, this rapid acid production in buffalo milk can lead to increased calcium loss, as the rising acidity dissolves calcium phosphate, which is then released into the whey and removed during curd formation

Keywords : Milk, Lactic Acid, Curd, Temperature, Calcium.

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Paper Submission Last Date
31 - December - 2025

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