Control of Pest Insect of June Beetle (Amphimallon solstitialis), with Entomopathogenic Nematodes


Authors : Nona Mikaia

Volume/Issue : Volume 9 - 2024, Issue 12 - December

Google Scholar : https://tinyurl.com/326c6zwb

Scribd : https://tinyurl.com/ykpwmdhc

DOI : https://doi.org/10.5281/zenodo.14591130

Abstract : The June beetle (Amphimallon solstitialis), a significant pest that affects grass, vegetables, and ornamental plants, causes substantial damage through its root-feeding larvae and foliage-feeding adults. This study evaluates the use of entomopathogenic nematodes as biological control agents against the fourth instar larvae of the June beetle. Two nematode species, Heterorhabditis bacteriophora and a local strain, Steinernema tsagveriensis sp.n., were tested for efficacy at various concentrations (2000 and 3500 infective juveniles per milliliter) and temperatures (25°C and 35°C). Results showed that H. bacteriophora achieved a mortality rate of 78%, while S. tsagveriensis sp.n. resulted in a mortality rate of 56%. The optimal conditions for nematode application included a suspension concentration of at least 2000 infective juveniles per milliliter and a temperature of 25°C. These findings underscore the effectiveness of both nematode species as ecologically sustainable solutions for controlling A. solstitialis, with H. bacteriophora demonstrating higher efficacy.

Keywords : Heterorhabditis Bacteriophora, Amphimallon Solstitialis, Biological Control, Temperature. Concentration of Nematode Suspension.

References :

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The June beetle (Amphimallon solstitialis), a significant pest that affects grass, vegetables, and ornamental plants, causes substantial damage through its root-feeding larvae and foliage-feeding adults. This study evaluates the use of entomopathogenic nematodes as biological control agents against the fourth instar larvae of the June beetle. Two nematode species, Heterorhabditis bacteriophora and a local strain, Steinernema tsagveriensis sp.n., were tested for efficacy at various concentrations (2000 and 3500 infective juveniles per milliliter) and temperatures (25°C and 35°C). Results showed that H. bacteriophora achieved a mortality rate of 78%, while S. tsagveriensis sp.n. resulted in a mortality rate of 56%. The optimal conditions for nematode application included a suspension concentration of at least 2000 infective juveniles per milliliter and a temperature of 25°C. These findings underscore the effectiveness of both nematode species as ecologically sustainable solutions for controlling A. solstitialis, with H. bacteriophora demonstrating higher efficacy.

Keywords : Heterorhabditis Bacteriophora, Amphimallon Solstitialis, Biological Control, Temperature. Concentration of Nematode Suspension.

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