Authors : Alma Tasia; Utmi Arma; Abu Bakar; Yenita Alamsyah; Dina Auliya Amly

Volume/Issue : Volume 10 - 2025, Issue 1 - January

Google Scholar : https://tinyurl.com/4bftc6n5

Scribd : https://tinyurl.com/2uzwbnmj

DOI : https://doi.org/10.5281/zenodo.14881333

Abstract : Oral Squamous Cell Carcinoma (OSCC) is one of the most aggressive malignant tumors due to its tendency to spread and high recurrence rate. Sea cucumbers have compounds that show anti- microbial, anti- inflammatory, and anticancer effects. The aim of this study was to determine the anticancer activity extract ethanol black sea cucumber ( Holothuria) atra ) against HSC-3 cell line in vitro. Extract black sea cucumber ( Holothuria) atra ) is done with the type of extraction maceration, then continued with u test Cytotoxicity by MTT Assay method using extract ethanol black sea cucumber ( Holothuria) atra ) at concentrations of 240 μg / ml, 120 μg / ml, 100 μg / ml, 80 μg / ml, 40 μg / ml, 20 μg / ml, 10 μg / ml, 5 μg / ml. At a concentration of 240 μg / ml has a cell viability of 35.215%, doxorubicin has a cell viability of 66.518%. The conclusion of this study is that there is cytotoxicity of ethanol extract of black sea cucumber ( Holothuria atra ) against HSC-3 cell line with IC50 value obtained of 41,371 μ g/ml using ethanol extract of Holothuria atra with concentration of 240 μ g/ml, 120 μ g/ml, 100 μ g/ml, 80 μ g/ml, 40 μ g/ml, 20 μ g/ml, 10 μ g/ml, 5 μ g/ml. At a concentration of 240 μg /ml there is a cell viability of 35,215%, this value is in the moderate toxicity category compared to the positive control of doxorubicin 0.1 μg /ml with a cell viability of 66,518% so that the extract ethanol black sea cucumber (Holothuria) atra ) has the potential to be an alternative to anticancer drugs.

Keywords : Oral Squamous Cell Carcinoma (OSCC), Black Sea Cucumber ( Holothuria atra ), U Test Toxicity, MTT Assay.

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