Authors : Ajay Bagherwal; Dr. Maya Sharma; Dr. Mrunal K. Shirsat

Volume/Issue : Volume 11 - 2026, Issue 4 - April

Google Scholar : https://tinyurl.com/jbdtppak

Scribd : https://tinyurl.com/33c9wfaa

DOI : https://doi.org/10.38124/ijisrt/26apr1890

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Abstract : A broad spectrum of triazole derivatives incorporating heterocyclic ring systems has been investigated for their potential as therapeutic agents, owing their biological properties, such as antifungal, antimicrobial, antibacterial, antiinflammatory, anticonvulsant action. The growing challenge of antimicrobial resistance demands the discovery of new compounds with differentiated chemical structures. In this study, a series of 2-(Dimethyl-amino)-N-(3-Mercapto-5-Phenyl-4H-1,2,4-triazol-4-yl) acetamide derivatives were synthesized. The synthetic pathway involved the conversion of aromatic acids into their corresponding esters, followed by reaction with hydrazine hydrate to form hydrazides. On further reaction with alcoholic KOH and carbon disulfide, the intermediates were converted into their corresponding potassium dithiocarbazinates. Subsequent Cyclization was carried out using hydrazine hydrate resulted in the formation of triazole derivatives. These compounds were then reacted with chloroacetyl chloride and finally with suitable amines to obtain the target molecules. Structural elucidation of the compounds was performed by nuclear magnetic resonance, infrared, and mass spectrometric analyses. The antimicrobial activities of the compounds (NJ-01, NJ-02, and NJ-03) were evaluated through the agar diffusion technique at concentrations spanning, from 1000 to 31.25 µg/ml, was employed through systematic dilution. Screening was carried out on Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) organisms using a disk diffusion assay. The compounds showed strong antibacterial effect on Gram-positive organism’s strains and promising antifungal activity effect on Candida albicans.

Keywords : Triazole, Benzoic Acid, Antibacterial Activity, Antifungal Activity and Staphylococus Aureus, Candida Albicans.

References :

1. Tsukuda T, Shiratori Y, Watanabe M, Ontsuka H, Hattori K, Shirai M, et al. Modeling, synthesis and biological activity of novel antifungal agents (1) Bioorg Med Chem Lett. 1998; 8:1819–24. 
2. Küçükgüzel I, Güniz Küçükgüzel S, Rollas S, Otük-Sanis G, Ozdemir O, Bayrak I, et al. Synthesis of some 3-(arylalkylthio)-4-alkyl/aryl-5-(4-aminophenyl)-4H-1,2,4-triazole derivatives and their anticonvulsant activity. Farmaco. 2004; 59:893–901.
3. Husain MI, Amir M. Synthesis of some new substituted thiosemicarbazides and triazoles as possible anticonvulsants. J Indian Chem Soc. 1986; 63:317–9. 
4. Valentina P, Ilango K, Deepthi M, Harusha P, Pavani G, Sindhura KL, et al. Antioxidant activity of some substituted 1,2,4-triazo-5-thione Schiff base. J Pharm Sci Res. 2009; 1:74–7. 
5. Sahin G, Palaska E, Kelicen P, Demirdamar R, Altinok G. Synthesis of some new 1-acylthiosemicarbazides, 1,3,4-oxadiazoles, 1,3,4-thiadiazoles and 1,2,4-triazole-3-thiones and their anti-inflammatory activities. Arzneimittelforschung. 2001; 51:478–84. 
6. Shivarama Holla B, Sooryanarayana Rao B, Sarojini BK, Akberali PM, Suchetha Kumari N. Synthesis and studies on some new fluorine containing triazolothiadiazines as possible antibacterial, antifungal and anticancer agents. Eur J Med Chem. 2006; 41:657–63.
7. Fridkin SK, Jarvis WR. Epidemiology of nosocomial fungal infections. Clin Microbiol Rev. 1996; 9:499–511. 
8. Alexander BD, Perfect JR. Antifungal resistance trends towards the year 2000. Implications for therapy and new approaches. Drugs. 1997; 54:657–78. 
9. Sauleau A, Sauleau J. Phosphorus Sulfur Silicon. 2000; 167:219. 
10. Srivastava, S.K.; Srivastava S.; Srivastava, S.D., Ind. J. Chem. 2002, 41B, , 1937.
11. Mishra, R.K.; Tiwari, R.K.; Shrivastava, S.; Bahel, S.C., J. Indian. Chem. Soc., 1991, 68, 110-112.
12. Zamini, K.; Faghihi K.; Tofighi, T.; Shariatzadeh, M.R., Turk J. Chem., 2004, 28, 95-100.