Pulmonary tuberculosis is the one of the main health
problem among Sudanese diabetic patients who are
considered as immune-compromised patients.
Objectives of this study
To evaluate the different cytokines among study
groups ( diabetic and tuberculosis patients, non diabetic
tuberculosis patients, diabetic and cardio vascular
disease patients and healthy individuals). And detection
of Mycobacterium tuberculosis using Real Time PCR.
This study was designed as descriptive a
prospective, analytical case-control study. This study was
enrolled 402 individuals’ 40 were diabetic and
tuberculosis patients, 41 non diabetic tuberculosis ,
diabetic CVD were 160 and 161 healthy control. Five ml
of blood withdraw in plain vaccutainor after informed
consent from individuals whom participate in this study .
We measured the cytokines,(IL6 ,IL10 and TNFα) levels
from all participant using direct ELISA technique . Then
the Real time PCR was done for all participants.
The mean levels of IL6 was recorded in 161 healthy
persons 94.80. while recorded 196.27 in 41 tubercles non
diabetic patients 210.27. in 40 tuberculous diabetic
patients and 297.42 . in 160 patients with CVD, TB and
diabetic199.33. The mean levels of IL10 was recorded in
161 healthy persons mean 4.21. while recorded mean 8.84
in 41 tubercles non diabetic patients , mean 8.61 in 40
tuberculous diabetic patients and mean 7.90 in 160
patients with CVD, TB and diabetic.
The mean levels of TNFα was 81.59 pg/ml in 161
healthy persons. while it was 232.29 pg/ml in 40 tubercles
non diabetic patients , 261,78 in 40 tuberculosis diabetic
patients and 297.42 in 160 patients with CVD, TB and
diabetic. Real time PCR was positive among T.B patients
and it was strong in 192 patients group ,while it was
negative It was a weak positive in 49 patients and
negative in 10 cardio vasulour disease. It was negative in
all healthy control 161 individules.
TNF was elevated in all groups of tuberculosis
patients regardless they were diabetic or not. All T.B
patients showed positive result by Real time PCR .63 %
from CVD with T.B and DM showed positive result. The
estimation of cytokines with combination with real time
PCR showed strong correlation.