Authors : Dr. Alaye T. T.; Kanda R.; Dr. Chaudhary A. J.; Otuku I. E.

Volume/Issue : Volume 11 - 2026, Issue 1 - January

Google Scholar : https://tinyurl.com/yy2wcwds

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DOI : https://doi.org/10.38124/ijisrt/26jan1191

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : The Niger Delta region of Nigeria has experienced persistent hydrocarbon contamination due to extensive oil and gas exploration activities. This study determined of total petroleum hydrocarbon (TPH) detection in hydrocarbon contaminated surface waters in the Niger Delta; using ELISA as an analytical technique. Ninety (90) surface water samples were collected from six (6) sampling locations, including a control site, and analyzed using the ELISA technique. Results revealed that TPH concentrations in surface water ranged from 0.033 to 10.32 mg/L, with an average of 3.49 ± 2.64 mg/L. These concentrations exceeded the Nigerian Standard for Drinking Water Quality (NSDWQ) limit of 0.003 mg/L and the Environmental Guidelines and Standards for the Petroleum Industry in Nigeria (EGASPIN) limit of 0.05 mg/L, indicating severe hydrocarbon pollution. The study concluded that ELISA method demonstrated high accuracy, sensitivity, and reproducibility, validating its efficiency as an alternative to conventional chromatographic methods for environmental hydrocarbon analysis.

Keywords : Hydrocarbon Pollution, TPH Concentration Determination, ELISA Technique, ERT Technique, Niger Delta Region, Nigeria.

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