Authors :
Faiqa Mubeen; Amna Mehmood; Ayesha Sajjad; Mehreen Mushtaq; Faryal Javaid; Sana Ullah Khan6; Maria Aslam; Usama Rehman
Volume/Issue :
Volume 8 - 2023, Issue 2 - February
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://bit.ly/3z8zDPu
DOI :
https://doi.org/10.5281/zenodo.7771024
Abstract :
To establish the importance of
immunohistochemical staining of P53 in grading of
Urothelial Carcinoma.
Methodology
A retrospective cross-sectional study was carried
out at the department of Histopathology, Lady Reading
Hospital (Medical Teaching Institution), Peshawar, from
August 2021 till February 2022. 97 Paraffin embedded
blocks of Urothelial carcinoma along with clinical record
of these patients, from January 2018 till December 2020,
were retrieved from data bank of Histopathology
department and Health management information
system. Cutting of blocks and staining with Hematoxylin
and Eosin (H&E) stain and P53 antibody was done.
Expression of p53 was noted by two consultant
pathologists. Nuclear immunoreactivity of strong
intensity was considered positive, if present in more than
10% of tumor cells, and negative if either no staining or
staining in less than 10% of tumor cells was noted.
Statistical analysis (Pearsmann correlation) was used to
determine the correlation among various variables.
Results
Out of 97 patients, the minimum age was 20 years
while maximum age was found to be 90 years with mean
+ standard deviation of 64 +11 years. There were 86
(88.6%) male patients and 11 (11.3) female patients.
49(51%) cases were low grade Urothelial carcinoma
whereas 48(49%) cases displayed high grade
morphology. p53 was found positive in 63(64.95%)
patients. Among positive cases, 45 cases were high grade
and 18 were low grade Urothelial carcinoma.
Conclusion
P53 Positivity was seen in 64.95% patients with
Urothelial carcinoma. P53 is an important
immunohistochemical marker for early diagnosis and
grading of Urothelial carcinoma cases
Keywords :
P53, Urothelial Carcinoma, Immunohistochemistry.
To establish the importance of
immunohistochemical staining of P53 in grading of
Urothelial Carcinoma.
Methodology
A retrospective cross-sectional study was carried
out at the department of Histopathology, Lady Reading
Hospital (Medical Teaching Institution), Peshawar, from
August 2021 till February 2022. 97 Paraffin embedded
blocks of Urothelial carcinoma along with clinical record
of these patients, from January 2018 till December 2020,
were retrieved from data bank of Histopathology
department and Health management information
system. Cutting of blocks and staining with Hematoxylin
and Eosin (H&E) stain and P53 antibody was done.
Expression of p53 was noted by two consultant
pathologists. Nuclear immunoreactivity of strong
intensity was considered positive, if present in more than
10% of tumor cells, and negative if either no staining or
staining in less than 10% of tumor cells was noted.
Statistical analysis (Pearsmann correlation) was used to
determine the correlation among various variables.
Results
Out of 97 patients, the minimum age was 20 years
while maximum age was found to be 90 years with mean
+ standard deviation of 64 +11 years. There were 86
(88.6%) male patients and 11 (11.3) female patients.
49(51%) cases were low grade Urothelial carcinoma
whereas 48(49%) cases displayed high grade
morphology. p53 was found positive in 63(64.95%)
patients. Among positive cases, 45 cases were high grade
and 18 were low grade Urothelial carcinoma.
Conclusion
P53 Positivity was seen in 64.95% patients with
Urothelial carcinoma. P53 is an important
immunohistochemical marker for early diagnosis and
grading of Urothelial carcinoma cases
Keywords :
P53, Urothelial Carcinoma, Immunohistochemistry.
