Authors : Sagar K.C; Krishma Paudel; Prajol Paudel; Navaraj Khadka; Sanjeet Khadka; Hasina Ranjitkar; Amrita Pantha; Anjila Paudyal; Swyika K.C; Samira Shrestha; Sunita Satshankar; Surakshya Kharel; Monika Sah; Praj Manandhar; Sampurna Karki; Priyanka Kumari Chaudhary Mandal; Bijaya Dahal; Sunil Gautam

Volume/Issue : Volume 9 - 2024, Issue 10 - October

Google Scholar : https://tinyurl.com/vrpwaxk4

Scribd : https://tinyurl.com/4a6xdf4b

DOI : https://doi.org/10.38124/ijisrt/IJISRT24OCT217

Abstract : Introduction: A key component in the prevention, management, and decline of healthcare-acquired illnesses is hand sanitizer. Multi-drug-resistant infections are among the persistent issues related to infections acquired while receiving medical care. Maintaining good hand hygiene can greatly lower the chance of cross-contamination. In recent times, hand sanitization has been increasingly popular as a means of reducing nosocomial infections that a variety of opportunistic bacteria can cause.  Method: Using a methanol solvent and a maceration method, the extract was obtained from the leaves of the neem and tulsi plants. The disc diffusion technique was used to determine and assess the antibacterial activity by measuring the diameter of the zone of inhibition. It was decided to create a herbal hand sanitizer.  Results: According to the study, both Ocimum sanctum and Azadirachta indica include terpenoids, alkaloids, glycosides, tannins, and saponins. All of the studied microorganisms displayed a zone of inhibition in the solvent extract within the 5-23 mm range. It was discovered that the extracts' antibacterial efficacy depended on concentration.  Conclusion: As a platform for further research and the creation of novel therapeutic entities, the study's findings indicate the presence of various phytochemical elements with antibacterial capabilities. We may infer that the stated organisms (S. aureus, K. pneumoniae, P. aeruginosa, & E. coli) are much less susceptible to bacterial growth when exposed to herbal sanitizer. Based on many anti-bacterial tests, it has been shown that the high-concentration formulation (40 mg/ml) is highly efficient in treating bacterial disorders.

Keywords : Hand Hygiene, Herbal Sanitizer, Antibacterial, Ocimum Sanctum, Azadirachta Indica.

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