Authors : Vijaya S.; Renuka G.; Anitha Devi U.; Parvathi D.; Ugandhar T.

Volume/Issue : Volume 11 - 2026, Issue 9 - September

Google Scholar : https://tinyurl.com/4fjkau2j

DOI : https://doi.org/10.38124/ijisrt/26sep419

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Tomatoes are one of the most significant vegetable crops grown worldwide due to their nutritional worth and economic relevance. Continuous genetic progress necessitates the use of dependable molecular techniques to measure genetic diversity and aid breeding efforts. Microsatellites, also known as simple sequence repeat (SSR) markers, are PCR-based, highly polymorphic, co-dominant markers that are frequently employed in plant genetic studies. The current work produced and characterised new SSR markers in tomato utilising a variety of farmed and wild accessions. Genomic DNA was extracted using a modified CTAB technique, and SSR loci were discovered by genome screening and primer design. The proposed markers generated unambiguous and consistent amplification patterns while revealing considerable allelic diversity between genotypes. These SSR markers are useful tools for genetic diversity studies, cultivar identification, and marker-assisted selection in tomato breeding efforts.

Keywords : SSR Markers, Genetic Diversity, Tomato, Solanum Lycopersicum, Molecular Breeding.

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