The objective of this research is to describe the optimization, validation, and application of chromatographic techniques for determination of Atorvastatin Calcium in their pharmaceutical formulation. In this work a simple, rapid, accurate and sensitive analytical methods have been developed and validated. This method is a direct spectro chromate graphic analytical method depend on the chromatographic separation of Atorvastatin calcium compound. This method was developed. By using C18 column with a mobile phase consisting of buffer solution and acetonitrile. The flow rate was adjusted at 1.5 ml/min, injection volume 20 μL, with UV-Detector the maximum absorption peak (λmax) at 238 nm. Column oven at ambient temperature, and retention time was found to be 4.405 min. Under the Optimized condition, beer’s law correlating the absorbance (Y) with concentration (X) was obeyed in the range of 2.0 to 35 μg/ml methods.