Development and Validation of a UV/vis Spectrometric Method for Determination of Ascorbic Acid in Pur State (Raw Material) and Dosage Forms

Authors : T. Mavanga; J. Mankulu; M. Mayangi; A. Mbenza; D. Mana; J. Mavar; JK. Mbinze

Volume/Issue : Volume 6 - 2021, Issue 4 - April

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A new and economical UV/vis spectrophotometric method using 0.1M hydrochloric acid as a dilution solvent for the estimation of vitamin C pure and in its dosage forms was developed in this work. The linearity study was performed over a range of concentrations from 2 to 12 µg / ml. The maximum absorption wavelength found was 243 nm. Linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, specificity / selectivity and robustness were chosen as criteria for validation of the method according to the requirements of the International Conference of 'Harmonization (ICH). The developed method was used to quantitatively determine vitamin C in its pharmaceutical forms marketed on the local market in the City of Kinshasa, DR Congo. The results obtained in this research demonstrate that the method is exact, precise, reproducible (% RSD <2%), simple, sensitive, robust, selective / specific and rapid.

Keywords : Ascorbic Acid, Spectrophotometry, UV/vis, Development, Validation.


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