Development and Validation of a UV/vis Spectrometric Method for Determination of Ascorbic Acid in Pur State (Raw Material) and Dosage Forms


Authors : T. Mavanga; J. Mankulu; M. Mayangi; A. Mbenza; D. Mana; J. Mavar; JK. Mbinze

Volume/Issue : Volume 6 - 2021, Issue 4 - April

Google Scholar : http://bitly.ws/9nMw

Scribd : https://bit.ly/3h9rxhx

Abstract : A new and economical UV/vis spectrophotometric method using 0.1M hydrochloric acid as a dilution solvent for the estimation of vitamin C pure and in its dosage forms was developed in this work. The linearity study was performed over a range of concentrations from 2 to 12 µg / ml. The maximum absorption wavelength found was 243 nm. Linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, specificity / selectivity and robustness were chosen as criteria for validation of the method according to the requirements of the International Conference of 'Harmonization (ICH). The developed method was used to quantitatively determine vitamin C in its pharmaceutical forms marketed on the local market in the City of Kinshasa, DR Congo. The results obtained in this research demonstrate that the method is exact, precise, reproducible (% RSD <2%), simple, sensitive, robust, selective / specific and rapid.

Keywords : Ascorbic Acid, Spectrophotometry, UV/vis, Development, Validation.

A new and economical UV/vis spectrophotometric method using 0.1M hydrochloric acid as a dilution solvent for the estimation of vitamin C pure and in its dosage forms was developed in this work. The linearity study was performed over a range of concentrations from 2 to 12 µg / ml. The maximum absorption wavelength found was 243 nm. Linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, specificity / selectivity and robustness were chosen as criteria for validation of the method according to the requirements of the International Conference of 'Harmonization (ICH). The developed method was used to quantitatively determine vitamin C in its pharmaceutical forms marketed on the local market in the City of Kinshasa, DR Congo. The results obtained in this research demonstrate that the method is exact, precise, reproducible (% RSD <2%), simple, sensitive, robust, selective / specific and rapid.

Keywords : Ascorbic Acid, Spectrophotometry, UV/vis, Development, Validation.

Never miss an update from Papermashup

Get notified about the latest tutorials and downloads.

Subscribe by Email

Get alerts directly into your inbox after each post and stay updated.
Subscribe
OR

Subscribe by RSS

Add our RSS to your feedreader to get regular updates from us.
Subscribe