To evaluate the standardized short regions of the genome of plant or animal species DNA barcoding technique was developed. This method is very useful to identify novel genes of known species. In online numerous sequence, databases are stored world-wide. To identify the species this is one of the methods used and it saves cost and time by omitting the sequencing step. The use of available barcode data to design optimized primers for further analysis, such as high- resolution melting analysis (HRM).This study identified the species that share similar external morphological features, using hybrid method Bar-HRM rather than conduct traditional taxonomic identification that requires major parts leaf, flower, the fruit of the specimens. Primer pairs derived from chloroplast regions (ITS1, ITS2, and rbcl) were used in the Bar- HRM. The method developed here was proven to be effective in distinguishing between the identification of different species in Piperaceae family plants. The extracted DNA from tested samples yielded a specific amplification product with the ITS1, ITS2 and rbcl primer. ITS1 region is proved to be the most promising universal DNA barcode for the plant family of Piperaceae.

Keywords : Piperaceae; Bar-HRM; rbcL; ITS2; ITS1; DNA Barcoding.