Porphyromonas gingivalis is a major
pathogen associated with initiation and progression of
periodontitis.
Aim and Objective
The aim of the study is to compare effect of lowlevel laser of wavelength 650nm on viability of
porphyromonas gingivalis with and without
photosensitisers.
Methods
Thirty samples of bacterial suspensions (200µl)
were prepared and divided into six groups .Group 1-
laser group (low level laser with wavelength of 650nm,
and irradiation time of 30 s), Group 2- Methylene blue +
laser group (after pre-irradiation time of 10 min, laser
was irradiated), and Group 3- Toluidine blue O + laser
group (after pre irradiation time of 10 min, LED was
irradiated), Group 4 -Methylene blue Group 5 -
Toluidine blue O (TBO) and Group 6- Control group
(no treatment), Then, 20 μL of each sample was cultured
in blood agar plates and incubated for 72 hours in
microaerophilic atmosphere for colony counting.
Result
The concentration of P. gingivalis was significantly
reduced after using the Methylene blue + laser and the
Toluidine blue O + laser group. (P values < 0.005)
Conclusion
Within the constraints of this investigation, it can
be deduced that photodynamic inactivation applying a
laser and photosensitizers like Methylene blue and
toluidine blue was more efficient than photosensitizers
and laser irradiation alone in eliminating P. gingivalis.
Keywords :
Porphyromonas Gingivalis , Low Level Laser , Methylene Blue , Toluidine Blue O.