Effect of Neem (Azadirachta indica) Leaf Aqueous Extract on Internode Length of Selected Cowpea Varieties Infested with Fusarium oxysporum


Authors : Falalu. M. I.; Yahaya. S. M.; Abubakar A. A.; Aisha. W. A.; Bakondi. H. H.; Zainab. Y. S.

Volume/Issue : Volume 11 - 2026, Issue 2 - February


Google Scholar : https://tinyurl.com/4mux9cja

Scribd : https://tinyurl.com/bdd2ju9m

DOI : https://doi.org/10.38124/ijisrt/26feb1167

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.


Abstract : Cowpea (Vigna unguiculata L.) is a major grain legume cultivated extensively in sub-Saharan Africa; however, its productivity is severely limited by soil-borne fungal pathogens, particularly Fusarium oxysporum. This study evaluated the effect of neem (Azadirachta indica) leaf aqueous extract on internode length of three cowpea varieties Sampe, Pampe 12, and a local cultivar infected with F. oxysporum. A pot experiment was conducted using a completely randomized design, where infected plants were treated with neem leaf aqueous extract, while untreated infected plants served as controls. Internode length was measured at defined growth stages, and data were subjected to analysis of variance. Application of neem extract significantly enhanced internode length compared with the untreated control, indicating a reduction in the deleterious effects of Fusarium infection. Varietal responses differed, with Pampe 12 exhibiting the greatest tolerance and growth recovery following treatment. The findings demonstrate the potential of neem leaf aqueous extract as an environmentally friendly alternative to synthetic fungicides for managing Fusarium wilt and improving vegetative growth in cowpea.

Keywords : Azadirachta indica; Vigna Unguiculata; Fusarium oxysporum; Botanical Fungicide; Internode Length; Disease Management.

References :

  1. Alemu, S. (2015). Cowpea (Vigna unguiculata (L.) Walp.) (Fabaceae) Landrace Diversity in southern Ethiopia [MSc Thesis]. Addis Ababa University.
  2. Ajani, O. O., Owoeye, T. F., and Akinmoladun, O. F. (2019). Antifungal activity of neem leaf extracts against Fusarium oxysporum f. sp. batatas. Asian Journal of Agricultural and Horticultural Research, 3(2), 1–9.
  3. Ajeigbe, H. A., Singh, B. B., and Musa, A. (2018). Breeding for improved disease resistance and yield in cowpea. Journal of Crop Improvement, 32(4), 450–465.
  4. Akinmoladun, O. F., Komolafe, T. R., andFarombi, O. (2019). Phytochemical screening and antimicrobial activity of neem (Azadirachta indica) leaf extracts. GSC Biological and Pharmaceutical Sciences, 6(3), 58–65.
  5. Alemu, M., Asfaw, Z., Woldu, Z., Fenta, B. A., andMedvecky, B. (2016). Cowpea (Vigna unguiculata (L.) Walp.) (Fabaceae) landrace diversity in northern Ethiopia. International Journal of Biodiversity and Conservation, 8(11), 297–309. https://doi.org/10.5897/IJBC2016. 0946.
  6. Akinbode, O. A., and Ikotun, T. (2008). Efficacy of certain plant extracts against seed-borne infection of Fusarium oxysporum in cowpea (Vigna unguiculata). African Journal of Biotechnology, 7(20), 3683–3685.
  7. Amadioha, A. C. (2000). Controlling rice blast in vitro and in vivo with extracts of Azadirachta indica. Crop Protection, 19(5), 287–290.
  8. Arogundade, O., Balogun, O. S., andFawole, O. B. (2007). Incidence and severity of common viral and fungal diseases of dry season tomato crop in a Southern Guinea Savannah Agro-ecology. Agrosearch9(1-2).
  9. Agrios, G. N. (2005). Plant pathology (5th ed.). Academic Press.
  10. Beckman, C. H. (1987). The nature of wilt diseases of plants. APS Press.
  11. Biswas, K., Chattopadhyay, I., Banerjee, R. K., andBandyopadhyay, U. (2002). Biological activities and medicinal properties of neem (Azadirachta indica). Current Science, 82(11), 1336–1345.
  12. Bhowmik, D., Chiranjib, Y. J., Tripathi, K. K., Kumar, S., andChandira, R. M. (2012). Herbal remedies of neem (Azadirachta indica) and its medicinal application. Journal of Chemical and Pharmaceutical Research, 4(1), 258–268.
  13. Baraka, M.A., Omar, S.A, El-Baraugy, Ebtehage, A.H., Zian 9 (2006). Controlling seedling damping – off, root – rot and wilt diseases of lupine (Lupinusalbus L.). AGRIC. Research Journal.Suez Canal Univ. 6(2006). Pp. 57 – 68.
  14. Bem, A.A, Anyam, R.W. and Iriga, O.S. (2010). Pathogens and toxins associated with wilting tomato in Makurdi: biotropic research international journal.Vol.2(1). Pp.1- 4
  15. Berendsen, R. L., Pieterse, C. M., and Bakker, P. A. (2012). The rhizosphere microbiome and plant health. Trends in plant science17(8), 478-486.
  16. Berendsen, R. L., Vismans, G., Yu, K., Song, Y., de Jonge, R., Burgman, W. P., and Pieterse, C. M. (2018). Disease-induced assemblage of a plant-beneficial bacterial consortium. The ISME journal12(6), 1496-1507.

Cowpea (Vigna unguiculata L.) is a major grain legume cultivated extensively in sub-Saharan Africa; however, its productivity is severely limited by soil-borne fungal pathogens, particularly Fusarium oxysporum. This study evaluated the effect of neem (Azadirachta indica) leaf aqueous extract on internode length of three cowpea varieties Sampe, Pampe 12, and a local cultivar infected with F. oxysporum. A pot experiment was conducted using a completely randomized design, where infected plants were treated with neem leaf aqueous extract, while untreated infected plants served as controls. Internode length was measured at defined growth stages, and data were subjected to analysis of variance. Application of neem extract significantly enhanced internode length compared with the untreated control, indicating a reduction in the deleterious effects of Fusarium infection. Varietal responses differed, with Pampe 12 exhibiting the greatest tolerance and growth recovery following treatment. The findings demonstrate the potential of neem leaf aqueous extract as an environmentally friendly alternative to synthetic fungicides for managing Fusarium wilt and improving vegetative growth in cowpea.

Keywords : Azadirachta indica; Vigna Unguiculata; Fusarium oxysporum; Botanical Fungicide; Internode Length; Disease Management.

Paper Submission Last Date
31 - March - 2026

SUBMIT YOUR PAPER CALL FOR PAPERS
Video Explanation for Published paper

Never miss an update from Papermashup

Get notified about the latest tutorials and downloads.

Subscribe by Email

Get alerts directly into your inbox after each post and stay updated.
Subscribe
OR

Subscribe by RSS

Add our RSS to your feedreader to get regular updates from us.
Subscribe