Thermo-alkaliphile bacteria derived from hot springs that have a pH above 8. Thermo-alkaliphilecurrently used as a source of various enzymes such as, lipase, protease, and amylase. Bacteria amylase is an enzyme that can hydrolyze starch into sugar. Amylase produced by thermo-alkaliphile bacteria role in industrial fields various, such as the leather tanning industry, biscuits, textiles, and as a detergent additive. In the detergent industry needed amylase resistant to temperature and high pH. Amylase needs in industry various increase up to 30% of the enzyme needs worldwide. Isolation of thermo-alkaliphile bacteria has been successfully carried out in a Semurup hot spring, Kerinci (Jambi) and obtained bacteria isolate thermo-alkaliphilel SMG9 with highest activity is 41.92 U/ml. The purpose of research is to determine the optimum temperature and pH of amylase activity through media optimization (temperature and pH). The results of the optimization of bacterial growth media temperature thermo-alkaliphile show that the highest amylase activity was obtained at 60°C. The results of the optimization of the bacterial growth medium pH thermoalkaliphile shows that the highest amylase activity was obtained at pH 8.5.

Keywords : amylase, optimization, activity assay, and thermoalkaliphilic bacteria.