Authors :
San Yu Myint; Soe Win; Tin Nwe Win; Kyi Moe
Volume/Issue :
Volume 8 - 2023, Issue 3 - March
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://bit.ly/4132ilq
DOI :
https://doi.org/10.5281/zenodo.7824493
Abstract :
Ethyl Methane Sulphonate (EMS) is a
chemical mutagen widely used to improve rice characters
by mutation breeding. The experiment was conducted to
find out the optimum lethal dose (LD50) of EMS on four
rice varieties viz., Aye Yar Min, Rice Berry, Jasmine 105
and Paw San (Black) for creating genetic variation. Dry
seeds of four rice varieties were directly treated with
different doses of EMS 0.00%, 0.25%, 0.50%, 0.75%,
1.00% and 1.25% for 22 hours. The effects of different
EMS doses and varieties and their interaction showed for
the traits like germination %, shoot length and root
length were significant differences. The lethal dose (LD50)
values were detected by regression analysis for different
varieties. The lethal dose (LD50) values for germination
%, shoot length and root length were observed at Aye
Yar Min (0.52 to 0.55%), Rice Berry (0.55 to 0.68%),
Jasmine 105 (0.57 to 0.60%) and Paw San (Black) (0.62 to
0.66%). The three studied traits were decreased with
increase in dose of EMS. The sensitivity of EMS appeared
to be related to the type of variety. The lethal dose (LD50)
values determined for the different rice varieties could be
useful while formulating future rice varietal improvement
breeding programmes.
Keywords :
Rice Varieties, Ethyl Methane Sulphonate (EMS), Lethal Dose (LD50) .
Ethyl Methane Sulphonate (EMS) is a
chemical mutagen widely used to improve rice characters
by mutation breeding. The experiment was conducted to
find out the optimum lethal dose (LD50) of EMS on four
rice varieties viz., Aye Yar Min, Rice Berry, Jasmine 105
and Paw San (Black) for creating genetic variation. Dry
seeds of four rice varieties were directly treated with
different doses of EMS 0.00%, 0.25%, 0.50%, 0.75%,
1.00% and 1.25% for 22 hours. The effects of different
EMS doses and varieties and their interaction showed for
the traits like germination %, shoot length and root
length were significant differences. The lethal dose (LD50)
values were detected by regression analysis for different
varieties. The lethal dose (LD50) values for germination
%, shoot length and root length were observed at Aye
Yar Min (0.52 to 0.55%), Rice Berry (0.55 to 0.68%),
Jasmine 105 (0.57 to 0.60%) and Paw San (Black) (0.62 to
0.66%). The three studied traits were decreased with
increase in dose of EMS. The sensitivity of EMS appeared
to be related to the type of variety. The lethal dose (LD50)
values determined for the different rice varieties could be
useful while formulating future rice varietal improvement
breeding programmes.
Keywords :
Rice Varieties, Ethyl Methane Sulphonate (EMS), Lethal Dose (LD50) .
